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Literature summary for 1.1.3.4 extracted from

  • Bankar, S.B.; Bule, M.V.; Singhal, R.S.; Ananthanarayan, L.
    Glucose oxidase - an overview (2009), Biotechnol. Adv., 27, 489-501.
    View publication on PubMed
Search for more literature for 1.1.3.4

Application

Application Comment Organism
diagnostics used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine Aspergillus niger
food industry food and beverage additive, used for low alcohol wine production, used for glucose removal from dried egg, improvement of color, flavor, and shelf life of food materials, oxygen removal from fruit juices, canned beverages, and from mayonnaise to prevent rancidity, used as ingredient of toothpaste, for the production of gluconic acid, and as a food preservative Aspergillus niger
synthesis GOD is used as a commercial source of gluconic acid, which can be produced by the hydrolysis of delta-glucono-1, 5-lactone, the endproduct of GOD catalysis Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate
-
 Aspergillus niger
Ag+
-
 Aspergillus niger
Cu2+
-
 Aspergillus niger
dimedone
-
 Aspergillus niger
Hg2+
-
 Aspergillus niger
hydrazine
-
 Aspergillus niger
hydroxylamine
-
 Aspergillus niger
phenylhydrazine
-
 Aspergillus niger
sodium bisulfate
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33
-
 beta-D-glucose
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
152000
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ion exchange chromatography, and gel filtration Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Storage Stability

Storage Stability Organism
-20°C, lyophilised enzyme, minimum of 6 months, remains stable Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + O2 + H2O low GOD activity Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 + H2O GOD is highly specific for the beta-anomer of D-glucose Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ir
D-galactose + O2 + H2O low GOD activity Aspergillus niger ?
-
 ?
D-mannose + O2 + H2O low GOD activity Aspergillus niger ?
-
 ?
additional information alpha-D-glucose is not a suitable substrate Aspergillus niger ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-D-glucose:oxygen 1-oxidoreductase
-
 Aspergillus niger
GOD
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 60
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 GOD has half-life of approximately 30 min at 37°C, immobilized GOD is more effective for applications at 37°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5
-
 Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Aspergillus niger