Application | Comment | Organism |
---|---|---|
medicine | analysis of the chronic inflammatory skin disorder Atopic eczema (AE) | Malassezia sympodialis |
Cloned (Comment) | Organism |
---|---|
recombinant protein, expressed in Escherichia coli strain BL21, pET17b expression vector | Malassezia sympodialis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | choline, glucose, myo-inositol, methanol, ethanol, 1-pentanol, benzyl alcohol, 2-phenylethanol, cholesterol or lauryl alcohol tested as potential substrates, recombinant protein devoid of either oxidase or dehydrogenase activity | Malassezia sympodialis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malassezia sympodialis | - |
strain ATCC 42132 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, gel filtration | Malassezia sympodialis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
sequence similarity to the glucose-methanol-choline (GMC) oxidoreductase enzyme superfamily, biophysical properties shown, function as an electron transport protein, no enzyme activity of the recombinant protein, major allergen in Atopic eczema patients | Malassezia sympodialis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | characterization of the allergen Mala s12, sequence similarity to glucose-methanol-choline (GMC) oxidoreductase enzyme superfamily, no enzyme activity of the recombinant protein in oxidase or dehydrogenase assay determined | Malassezia sympodialis | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound to the recombinant protein of Mala s12, flavin content per monomer of protein calculated | Malassezia sympodialis |