Application | Comment | Organism |
---|---|---|
analysis | the enzyme might by useful in designing of biosensors for use in clinical, biochemical, and diagnostic assays | Penicillium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.4 | - |
beta-D-glucose | pH 7.0, 25°C | Penicillium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | isozyme 2 | Penicillium sp. | - |
- |
intracellular | isozyme 1 | Penicillium sp. | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Penicillium sp. |
148000 | - |
gel filtration | Penicillium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 | Penicillium sp. | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 | Penicillium sp. CBS 120262 | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium sp. | - |
two isozymes | - |
Penicillium sp. CBS 120262 | - |
two isozymes | - |
Purification (Comment) | Organism |
---|---|
native enzyme 63fold by ammonium sulfate fractionation, ultrafiltration, anion exchange chromatography, and gel filtration, to 93% purity | Penicillium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.08 U/mg dry weight for the intracellular enzyme, and 6.9U/ml for the extracellular enzyme | Penicillium sp. |
240.5 | - |
purified enzyme | Penicillium sp. |
Storage Stability | Organism |
---|---|
the lyophilized purified enzyme is stable at -20 °C for a minimum of 6 months. | Penicillium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 | - |
Penicillium sp. | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 | via cofactor FAD reduction to FADH2, reaction cycles, FADH2 reduces O2, overview | Penicillium sp. | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 | - |
Penicillium sp. CBS 120262 | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 | via cofactor FAD reduction to FADH2, reaction cycles, FADH2 reduces O2, overview | Penicillium sp. CBS 120262 | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Penicillium sp. |
Synonyms | Comment | Organism |
---|---|---|
GOX | - |
Penicillium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Penicillium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | 70% of maximal activity at 10°C and 45°C | Penicillium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
10 h, purified enzyme, stable | Penicillium sp. |
37 | - |
half-life: 30 min, purified enzyme | Penicillium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
741 | - |
beta-D-glucose | pH 7.0, 25°C | Penicillium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Penicillium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.9 | 8.9 | 70% of maximal activity at pH 4.9 and pH 8.9 | Penicillium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Penicillium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penicillium sp. | intracellular isozyme 1 | - |
4.3 |
Penicillium sp. | extracellular isozyme 2 | - |
4.7 |