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Literature summary for 1.1.3.4 extracted from
- Purification and thermodynamic characterization of glucose oxidase from a newly isolated strain of Aspergillus niger (2006), Can. J. Microbiol., 52, 519-524.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is useful in designing of biosensors for use in clinical, biochemical, and diagnostic assays | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | thermodynamics | Aspergillus niger | |
| 25 | - |
D-glucose | pH 5.5, 40°C | Aspergillus niger |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | Aspergillus niger NFCCP | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger NFCCP | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 135 | - |
purified enzyme | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | highly substrate specific enzyme | Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | - |
Aspergillus niger NFCCP | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-glucose + O2 | highly substrate specific enzyme | Aspergillus niger NFCCP | D-glucono-1,5-lactone + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GOD | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, half-life: 30 min | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus niger |
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Release 2023.1 (January 2023)
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