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Literature summary for 1.1.3.4 extracted from
- Production, purification and characterization of glucose oxidase from a newly isolated strain of Penicillium pinophilum (1997), Appl. Microbiol. Biotechnol., 48, 34-40.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0368 | - |
2,6-dichloroindophenol | - |
Talaromyces pinophilus |  |
| 6.2 | - |
beta-D-glucose | - |
Talaromyces pinophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77700 | - |
2 * 77700, SDS-PAGE, confirmed by electron-microscopic examinations | Talaromyces pinophilus |
| 148000 | - |
gel filtration on Superdex 200 | Talaromyces pinophilus |
| 152800 | - |
electron microscopy | Talaromyces pinophilus |
| 159000 | - |
calculated value based on Stokes' radius and sedimentation coefficient | Talaromyces pinophilus |
| 160000 | - |
gel filtration on Sephacryl S-300 | Talaromyces pinophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces pinophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using an extraction step of the mycelium mass at pH 3.0, cation exchange chromatography on S-Sepharose and gel filtration on Sephacryl S-300 | Talaromyces pinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Talaromyces pinophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + 2,6-dichlorophenol indophenol | - |
Talaromyces pinophilus | D-glucono-1,5-lactone + ? | - |
? | |
| beta-D-glucose + O2 + H2O | specific for D-glucose, 2,6-dichloroindophenol can act as artificial electron acceptor | Talaromyces pinophilus | D-glucono-1,5-lactone + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 77700, SDS-PAGE, confirmed by electron-microscopic examinations | Talaromyces pinophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | in 100 mM potassium phosphate, pH 5.0 | Talaromyces pinophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at pH 2-4 and pH 7-8, half life: less than 1 day | Talaromyces pinophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Talaromyces pinophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
50% activity | Talaromyces pinophilus |
| 5 | - |
extremely stable, no loss of activity detected after 300 days at 4°C and 30°C | Talaromyces pinophilus |
| 6 | - |
extremely stable | Talaromyces pinophilus |
| 7.5 | - |
50% activity | Talaromyces pinophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1.76 nmol FAD per nmol enzyme | Talaromyces pinophilus | |
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