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Literature summary for 1.1.3.4 extracted from

  • Kalisz, H.M.; Hendle, J.; Schmid, R.D.
    Structural and biochemical properties of glycosylated and deglycosylated glucose oxidase from Penicillium amagasakiense (1997), Appl. Microbiol. Biotechnol., 47, 502-507.
    View publication on PubMed
Search for more literature for 1.1.3.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 beta-D-glucose 0.05 M Tris buffer, pH 8 Penicillium amagasakiense
2
-
 beta-D-glucose 0.1 M Tris buffer, pH 8 Penicillium amagasakiense
4.4
-
 beta-D-glucose 0.6 M sodium acetate buffer, pH 6 Penicillium amagasakiense
5
-
 beta-D-glucose 0.1 M sodium acetate buffer, pH 5 Penicillium amagasakiense
5.2
-
 beta-D-glucose 0.1 M sodium acetate buffer, pH 6 Penicillium amagasakiense
6.4
-
 beta-D-glucose 0.1 M sodium phosphate buffer, pH 7 Penicillium amagasakiense
6.7
-
 beta-D-glucose 0.1 M potassium phosphate buffer, pH 7 Penicillium amagasakiense
7.1
-
 beta-D-glucose 0.1 M sodium acetate buffer, pH 4.5 Penicillium amagasakiense
8.1
-
 beta-D-glucose 0.1 M potassium phosphate buffer, pH 6 Penicillium amagasakiense
16.5
-
 beta-D-glucose 0.1 M sodium acetate buffer, pH 4 Penicillium amagasakiense
22.5
-
 beta-D-glucose 0.6 M sodium acetate buffer, pH 4.5 Penicillium amagasakiense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
167000
-
 glycosylated enzyme, PAGE under non-dissociating conditions Penicillium amagasakiense
176000
-
 glycosylated enzyme, gel filtration Penicillium amagasakiense

Organism

Organism UniProt Comment Textmining
Penicillium amagasakiense
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme consists of 13% carbohydrate comprising 95 residues of mannose, 12 residues of glucosamine and 5 residues of galactose per molecule of enzyme, six N-glycosylation sites estimated for the dimer Penicillium amagasakiense

Purification (Commentary)

Purification (Comment) Organism
using ion-exchange chromatography on a Mono Q HR 5/5 column Penicillium amagasakiense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2 + H2O
-
 Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50 glycosylated and deglycosylated enzyme Penicillium amagasakiense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6.5 glycosylated and deglycosylated enzyme Penicillium amagasakiense

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 at 4°C, 7 months, glycine/HCl buffer, residual activity of the glycosylated enzyme: 34%, residual activity of the deglycosylated enzyme: 10% Penicillium amagasakiense
4
-
 at 4°C, 7 months, glycine/HCl buffer, residual activity of the glycosylated enzyme: 58%, residual activity of the deglycosylated enzyme: 30% Penicillium amagasakiense
4
-
 at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 65%, residual activity of the deglycosylated enzyme: 59% Penicillium amagasakiense
5
-
 at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 81%, residual activity of the deglycosylated enzyme: 60% Penicillium amagasakiense
6
-
 at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 76%, residual activity of the deglycosylated enzyme: 70% Penicillium amagasakiense
6
-
 at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 80%, residual activity of the deglycosylated enzyme: 75% Penicillium amagasakiense
7
-
 at 4°C, 7 months, Tris/HCl buffer, residual activity of the glycosylated enzyme: 71%, residual activity of the deglycosylated enzyme: 70% Penicillium amagasakiense
7
-
 at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 88%, residual activity of the deglycosylated enzyme: 87% Penicillium amagasakiense
8
-
 at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 7%, residual activity of the deglycosylated enzyme: 5% Penicillium amagasakiense
8
-
 at 4°C, 7 months, TRIS/HCl buffer, residual activity of the glycosylated enzyme: 20%, residual activity of the deglycosylated enzyme: 16% Penicillium amagasakiense
9
-
 at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 5%, residual activity of the deglycosylated enzyme: less than 5% Penicillium amagasakiense
9
-
 at 4°C, 7 months, Tris/HCl buffer, residual activity of the glycosylated enzyme: less than 2%, residual activity of the deglycosylated enzyme: less than 2% Penicillium amagasakiense
10
-
 at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1% Penicillium amagasakiense
11
-
 at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1% Penicillium amagasakiense
12
-
 at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1% Penicillium amagasakiense