Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibition is explained by the high reactivity of this ion for thiol groups essential for enzymic activity | Talaromyces variabilis | |
AgNO3 | 24% activity remains at 10 mM | Talaromyces variabilis | |
CuCl2 | 6.5% activity remains at 10 mM | Talaromyces variabilis | |
NaF | 72% activity remains at 10 mM | Talaromyces variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
beta-D-glucose | - |
Talaromyces variabilis | |
20 | - |
2-deoxy-D-glucose | - |
Talaromyces variabilis | |
33 | - |
D-xylose | - |
Talaromyces variabilis | |
44 | - |
D-mannose | - |
Talaromyces variabilis | |
117 | - |
D-maltose | - |
Talaromyces variabilis | |
162 | - |
D-fructose | - |
Talaromyces variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, SDS-PAGE | Talaromyces variabilis |
126000 | - |
gel filtration | Talaromyces variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces variabilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ion-exchange chromatography on DEAE-Sepharose and gel filtration chromatography on Sephacryl S-300 | Talaromyces variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Talaromyces variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + O2 + H2O | 19.6% relative activity to D-glucose | Talaromyces variabilis | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | highly specific | Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | glucose is the primary substrate for the enzyme | Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-fructose + O2 + H2O | 4.9% relative activity to D-glucose | Talaromyces variabilis | ? + H2O2 | - |
? | |
D-maltose + O2 + H2O | 21.3% relative activity to D-glucose | Talaromyces variabilis | ? + H2O2 | - |
? | |
D-xylose + O2 + H2O | 3% relative activity to D-glucose | Talaromyces variabilis | ? | - |
? | |
mannose + O2 + H2O | 7.2% relative activity to D-glucose | Talaromyces variabilis | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, SDS-PAGE | Talaromyces variabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Talaromyces variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
completely inactive | Talaromyces variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Talaromyces variabilis |