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Literature summary for 1.1.3.4 extracted from
- Immobilization of glucose oxidase with Bombyx mori silk fibroin by only stretching treatment and its application to glucose sensor (1989), Biotechnol. Bioeng., 33, 598-603.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | enzyme immobilized in Bombyx mori silk fibroin membrane applied to glucose sensor | Bombyx mori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
beta-D-glucose | immobilized enzyme, immobilized membrane with ratio stretching 1.25 | Bombyx mori |  |
| 4.5 | - |
beta-D-glucose | immobilized enzyme, methanol treated | Bombyx mori | |
| 5.4 | - |
beta-D-glucose | immobilized enzyme, immobilized membrane with ratio stretching 3 | Bombyx mori | |
| 25.2 | - |
beta-D-glucose | free enzyme | Bombyx mori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | Bombyx mori | - |
D-glucono-1,5-lactone + H2O2 | in a subsequent step D-glucono-1,5-lactone is nonenzymatically hydrolyzed to D-gluconic acid | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, immobilized, membrane in dried state, more than 12 months | Bombyx mori |
| 4°C, more than 90% activity retained, 100 mM phosphate buffer, pH 7, immobilized, 4 months | Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | - |
Bombyx mori | D-glucono-1,5-lactone + H2O2 | - |
? | |
| beta-D-glucose + O2 + H2O | - |
Bombyx mori | D-glucono-1,5-lactone + H2O2 | in a subsequent step D-glucono-1,5-lactone is nonenzymatically hydrolyzed to D-gluconic acid | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the activity of the free enzyme decreases to 30% of the original value, the activity of the immobilized enzyme scarcely decreases | Bombyx mori |
| 70 | - |
inmobilized enzyme, more than 60% of the activity remains | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7 | immobilized on silk fibroin | Bombyx mori |
| 5.7 | - |
soluble enzyme | Bombyx mori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Bombyx mori |
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