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Literature summary for 1.1.3.30 extracted from
- Purification and properties of polyvinyl alcohol oxidase with broad substrate range obtained from Pseudomonas vesicularis var. povalolyticus PH (1997), World J. Microbiol. Biotechnol., 13, 273-277.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 23% inhibition | Brevundimonas vesicularis |  |
| Fe2+ | 1 mM, 88% inhibition | Brevundimonas vesicularis | |
| Hg2+ | 1 mM, 93% inhibition | Brevundimonas vesicularis | |
| Sn2+ | 1 mM, 89% inhibition | Brevundimonas vesicularis | |
| Thiourea | 1 mM, 20% inhibition | Brevundimonas vesicularis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1 mol iron/mol enzyme | Brevundimonas vesicularis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Brevundimonas vesicularis |
| 85000 | - |
1 * 85000, SDS-PAGE | Brevundimonas vesicularis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyvinyl alcohol + O2 | Brevundimonas vesicularis | - |
oxidized polyvinyl alcohol + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas vesicularis | - |
var. povalolyticus PH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, AF blue Toyopearl, Q-Sepharose | Brevundimonas vesicularis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.7 | - |
- |
Brevundimonas vesicularis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-butanediol + O2 | 3.8% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 1,3-butanedione + H2O2 | - |
? | |
| 2,4-pentanediol + O2 | 19.3% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 2,4-pentanedione + H2O2 | - |
? | |
| 2,5-hexanediol + O2 | 3.8% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 2,5-hexanedione + H2O2 | - |
? | |
| 2-butanol + O2 | 47.3% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 2-butanone + H2O2 | - |
? | |
| 2-hexanol + O2 | 65.4% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 2-hexanone + H2O2 | - |
? | |
| 2-pentanol + O2 | 36.3% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 2-pentanone + H2O2 | - |
? | |
| 2-propanol + O2 | 3.5% of activity with polyvinyl alcohol | Brevundimonas vesicularis | acetone + H2O2 | - |
? | |
| 3-butanol + O2 | 14.6% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 3-butanone + H2O2 | - |
? | |
| 3-heptanol + O2 | 68.8% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 3-heptanone + H2O2 | - |
? | |
| 3-hexanol + O2 | 47.3% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 3-hexanone + H2O2 | - |
? | |
| 4-decanol + O2 | 100% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 4-decanone + H2O2 | - |
? | |
| 4-heptanol + O2 | 75.1% of activity with polyvinyl alcohol | Brevundimonas vesicularis | 4-heptanone + H2O2 | - |
? | |
| benzyl alcohol + O2 | 13.7% of activity with polyvinyl alcohol | Brevundimonas vesicularis | benzaldehyde + H2O2 | - |
? | |
| cyclohexanol + O2 | 41.8% of activity with polyvinyl alcohol | Brevundimonas vesicularis | cyclohexanal + H2O2 | - |
? | |
| polyvinyl alcohol + O2 | - |
Brevundimonas vesicularis | oxidized polyvinyl alcohol + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 85000, SDS-PAGE | Brevundimonas vesicularis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Brevundimonas vesicularis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Brevundimonas vesicularis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable for 30 min, complete loss of activity after 30 min at 60°C | Brevundimonas vesicularis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Brevundimonas vesicularis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | almost constant activity in this range | Brevundimonas vesicularis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable for at least 24 h at 30°C | Brevundimonas vesicularis |
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