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show all sequences of 1.1.3.29

Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp. 15-1

Horiuchi, T.; Agric. Biol. Chem. 53, 361-368 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
SDS
slightly accelerates activity
Pseudomonas sp.
Application
Application
Commentary
Organism
analysis
enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
weak
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
oxalate
-
Pseudomonas sp.
PCMB
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
0.24
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
2.6
-
N-Glycolyl-D-glucosamine
-
Pseudomonas sp.
10
-
D-galactosamine
-
Pseudomonas sp.
18
-
N,N-diacetylchitobiose
-
Pseudomonas sp.
33
-
N-acetyl muramic acid
-
Pseudomonas sp.
40
-
D-glucosamine
-
Pseudomonas sp.
40
-
N-acetyl-D-mannosamine
-
Pseudomonas sp.
59
-
D-mannose
-
Pseudomonas sp.
170
-
D-galactose
-
Pseudomonas sp.
290
-
D-glucose
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
36000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
44000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
63000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
170000
-
gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 15-1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
70.4
-
N-acetyl-D-glucosamine
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactosamine + O2
35% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-galactose + O2
0.43% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-galactono-1,5-lactone + H2O2
-
-
-
?
D-galactose + O2
0.43% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
D-galactono-1,5-lactone + H2O2
-
-
-
?
D-glucosamine + O2
14% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-glucose + O2
0.3% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-gluconolactone + H2O2
-
-
-
?
D-mannosamine + O2
0.03% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-mannose + O2
0.38% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-mannonolactone + H2O2
-
-
-
?
N,N-diacetylchitobiose + O2
31% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
N-acetyl-beta-D-glucosamine + O2
alpha-anomer is not oxidized
389762
Pseudomonas sp.
N-acetyl-D-glucosaminolactone + H2O2
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
389762
Pseudomonas sp.
?
N-acetyl-beta-D-glucosamine + O2
alpha-anomer is not oxidized
389762
Pseudomonas sp. 15-1
N-acetyl-D-glucosaminolactone + H2O2
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
389762
Pseudomonas sp. 15-1
?
N-acetyl-D-galactosamine + O2
98% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-acetyl-D-galactosaminolactone + H2O2
-
-
-
?
N-acetyl-D-galactosamine + O2
98% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-acetyl-D-galactosaminolactone + H2O2
-
-
-
?
N-acetyl-D-mannosamine + O2
4.9% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-acetyl-D-mannosaminolactone + H2O2
-
-
-
?
N-acetyl-D-mannosamine + O2
4.9% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-acetyl-D-mannosaminolactone + H2O2
-
-
-
?
N-acetylmuramic acid + O2
44% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
N-glycolyl-D-glucosamine + O2
72% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-glycolyl-D-glucosaminolactone + H2O2
-
-
-
?
N-glycolyl-D-glucosamine + O2
72% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-glycolyl-D-glucosaminolactone + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
10 min, stable
Pseudomonas sp.
70
-
100% remaining activity after 10 min
Pseudomonas sp.
83
-
50% remaining activity after 10 min
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
9
stable at 45C for 10 min
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
probably contains unidentified flavin component
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
SDS
slightly accelerates activity
Pseudomonas sp.
Application (protein specific)
Application
Commentary
Organism
analysis
enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
probably contains unidentified flavin component
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
weak
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
oxalate
-
Pseudomonas sp.
PCMB
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
0.24
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
2.6
-
N-Glycolyl-D-glucosamine
-
Pseudomonas sp.
10
-
D-galactosamine
-
Pseudomonas sp.
18
-
N,N-diacetylchitobiose
-
Pseudomonas sp.
33
-
N-acetyl muramic acid
-
Pseudomonas sp.
40
-
D-glucosamine
-
Pseudomonas sp.
40
-
N-acetyl-D-mannosamine
-
Pseudomonas sp.
59
-
D-mannose
-
Pseudomonas sp.
170
-
D-galactose
-
Pseudomonas sp.
290
-
D-glucose
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
36000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
44000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
63000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
170000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
70.4
-
N-acetyl-D-glucosamine
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactosamine + O2
35% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-galactose + O2
0.43% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-galactono-1,5-lactone + H2O2
-
-
-
?
D-galactose + O2
0.43% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
D-galactono-1,5-lactone + H2O2
-
-
-
?
D-glucosamine + O2
14% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-glucose + O2
0.3% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-gluconolactone + H2O2
-
-
-
?
D-mannosamine + O2
0.03% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
D-mannose + O2
0.38% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
D-mannonolactone + H2O2
-
-
-
?
N,N-diacetylchitobiose + O2
31% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
N-acetyl-beta-D-glucosamine + O2
alpha-anomer is not oxidized
389762
Pseudomonas sp.
N-acetyl-D-glucosaminolactone + H2O2
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
389762
Pseudomonas sp.
?
N-acetyl-beta-D-glucosamine + O2
alpha-anomer is not oxidized
389762
Pseudomonas sp. 15-1
N-acetyl-D-glucosaminolactone + H2O2
in presence of H2O: N-acetyl-D-glucosaminate + H2O2
389762
Pseudomonas sp. 15-1
?
N-acetyl-D-galactosamine + O2
98% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-acetyl-D-galactosaminolactone + H2O2
-
-
-
?
N-acetyl-D-galactosamine + O2
98% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-acetyl-D-galactosaminolactone + H2O2
-
-
-
?
N-acetyl-D-mannosamine + O2
4.9% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-acetyl-D-mannosaminolactone + H2O2
-
-
-
?
N-acetyl-D-mannosamine + O2
4.9% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-acetyl-D-mannosaminolactone + H2O2
-
-
-
?
N-acetylmuramic acid + O2
44% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
?
-
-
-
?
N-glycolyl-D-glucosamine + O2
72% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp.
N-glycolyl-D-glucosaminolactone + H2O2
-
-
-
?
N-glycolyl-D-glucosamine + O2
72% of the activity with N-acetyl-D-glucosamine
389762
Pseudomonas sp. 15-1
N-glycolyl-D-glucosaminolactone + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE
Pseudomonas sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
10 min, stable
Pseudomonas sp.
70
-
100% remaining activity after 10 min
Pseudomonas sp.
83
-
50% remaining activity after 10 min
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
9
stable at 45C for 10 min
Pseudomonas sp.
Other publictions for EC 1.1.3.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389762
Horiuchi
-
Purification and properties of ...
Pseudomonas sp., Pseudomonas sp. 15-1
Agric. Biol. Chem.
53
361-368
1989
1
1
-
-
-
-
8
11
-
-
5
-
-
2
-
-
1
-
-
-
1
-
17
1
-
-
3
-
1
1
1
1
-
-
-
1
1
-
1
-
-
-
-
8
-
11
-
-
5
-
-
-
-
1
-
-
1
-
17
1
-
-
3
-
1
1
1
-
-
-
-
-
-
-