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Literature summary for 1.1.3.21 extracted from

  • Li, Z.; Qiao, Y.; Cai, L.; Nakanishi, H.; Gao, X.D.
    Characterization of glycerol phosphate oxidase from Streptococcus pneumoniae and its application for ketose synthesis (2015), Bioorg. Med. Chem. Lett., 25, 504-507.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
dihydroxyacetone phosphate
-
Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-glycerol 3-phosphate + O2 Streptococcus pneumoniae
-
dihydroxyacetone phosphate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-glycerol 3-phosphate + O2
-
Streptococcus pneumoniae dihydroxyacetone phosphate + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
glycerol phosphate oxidase
-
Streptococcus pneumoniae
GPO
-
Streptococcus pneumoniae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Streptococcus pneumoniae

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 60 the relative activity at 50┬░C remains more than 60% of the maximum activity. The enzyme almost loses its activity at 60┬░C Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptococcus pneumoniae