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Literature summary for 1.1.3.21 extracted from

  • Hames, C.; Halbedel, S.; Hoppert, M.; Frey, J.; Stuelke, J.
    Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae (2009), J. Bacteriol., 191, 747-753.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information absence of substrate induction in the expression of the enzymes of glycerol metabolism Mycoplasma pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
gene glpD, DNA sequence determination of wild-type and transposon insertion mutant enzymes, expression analysis Mycoplasma pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information random transposon insertion mutations, construction of the glpD mutant strain GPM52, the glpD mutant exhibits a significantly reduced formation of hydrogen peroxide and a severely reduced cytotoxicity. GlpD disruption does not prevent synthesis of the essential protein GlpK Mycoplasma pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
additional information absence of carbon catabolite repression in the expression of the enzymes of glycerol metabolism Mycoplasma pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the major fraction of GlpD is present in the cytoplasm Mycoplasma pneumoniae 5737
-
membrane membrane-spanning protein Mycoplasma pneumoniae 16020
-
membrane a minor fraction of GlpD is associated with the membrane Mycoplasma pneumoniae 16020
-
additional information subcellular localization analysis, overview Mycoplasma pneumoniae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol 3-phosphate + O2 Mycoplasma pneumoniae involved in the glycerate metabolism dihydroxyacetone phosphate + H2O2
-
?
glycerol 3-phosphate + O2 Mycoplasma pneumoniae M129 involved in the glycerate metabolism dihydroxyacetone phosphate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Mycoplasma pneumoniae
-
gene glpD
-
Mycoplasma pneumoniae
-
-
-
Mycoplasma pneumoniae M129
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol 3-phosphate + O2
-
Mycoplasma pneumoniae dihydroxyacetone phosphate + H2O2
-
?
glycerol 3-phosphate + O2 involved in the glycerate metabolism Mycoplasma pneumoniae dihydroxyacetone phosphate + H2O2
-
?
glycerol 3-phosphate + O2
-
Mycoplasma pneumoniae M129 dihydroxyacetone phosphate + H2O2
-
?
glycerol 3-phosphate + O2 involved in the glycerate metabolism Mycoplasma pneumoniae M129 dihydroxyacetone phosphate + H2O2
-
?

Synonyms

Synonyms Comment Organism
GLPD
-
Mycoplasma pneumoniae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Mycoplasma pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycoplasma pneumoniae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mycoplasma pneumoniae

General Information

General Information Comment Organism
malfunction the glpD mutant strain GPM52 (glpD gene disrupted between nucleotides 555 and 556, resulting in a truncated protein of 185 amino acids) does not grow at all in glycerol-containing medium Mycoplasma pneumoniae
metabolism glycerol is one of the few carbon sources that can be utilized by Mycoplasma pneumoniae. Glycerol metabolism involves uptake by facilitated diffusion, phosphorylation, and the oxidation of glycerol 3-phosphate to dihydroxyacetone phosphate, a glycolytic intermediate. Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae with the enzyme being important but not essential, overview Mycoplasma pneumoniae
physiological function the formation of hydrogen peroxide by GlpD is crucial for cytotoxic effects of Mycoplasma pneumoniae. The glycerol 3-phosphate oxidase encoded by glpD is essential for glycerol utilization Mycoplasma pneumoniae