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Literature summary for 1.1.3.21 extracted from

  • Minaksh, M.; Pundir, C.
    Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum (2008), Indian J. Biochem. Biophys., 45, 111-115.
    View publication on PubMed

Application

Application Comment Organism
analysis co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum Aerococcus viridans

Protein Variants

Protein Variants Comment Organism
additional information co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum, reusability of the strip-bound enzyme, overview Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Aerococcus viridans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol 3-phosphate + O2 Aerococcus viridans
-
dihydroxyacetone phosphate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Aerococcus viridans
-

Storage Stability

Storage Stability Organism
immobilized enzyme, 50% loss of activity after 150 uses in analysis for triglycerides, within 33 days at 4┬░C in 0.1 M sodium phosphate buffer, pH 7.0 Aerococcus viridans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol 3-phosphate + O2
-
Aerococcus viridans dihydroxyacetone phosphate + H2O2
-
?
glycerol 3-phosphate + O2 from triolein, coupled assay method by use of co-immobilized lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase, overview Aerococcus viridans dihydroxyacetone phosphate + H2O2
-
?

Synonyms

Synonyms Comment Organism
GPO
-
Aerococcus viridans

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
immobilized enzyme Aerococcus viridans

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
70
-
15 min,immobilized enzyme, 50% remaining activity Aerococcus viridans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
immobilized enzyme Aerococcus viridans