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Literature summary for 1.1.3.21 extracted from

  • Aragon Casale, C.; Ferreira-Dias, S.; Gattas Aparecida de Lucca, E.; Maristela de Freitas Sanches, P.
    Characterization of glycerol kinase from bakers yeast: Response surface modeling of the enzymatic reaction (2008), J. Mol. Catal. B, 52-53, 113-120.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
4-aminoantipirine 0.009%, ranging from 0.006 to 0.074% Saccharomyces cerevisiae
phenol activity positively affected by the presence of 0.018% phenol, ranging from 0.006 to 0.123% Saccharomyces cerevisiae
Triton X-100 0.1% of Triton X-100 slightly stimulates enzyme activity Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
consumption of oxygen determined by oxygraphy, activity measured by peroxidase chromogen method, a 120 min reaction time and a concentration of 0.5 M of glycerol phosphate considered as best conditions for determination of enzyme activity Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
40┬░C, during storage for 21 days 20% of activity lost, sodium azide as stabilizer in a concentration of 0.05% maintains activity of the enzyme in 98.4%, addition of sucrose maintains only 74% of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol-3-phosphate + O2
-
Saccharomyces cerevisiae glycerone phosphate + H2O2
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60
-
at pH 8.0, tested at temperatures of 25, 30, 37, 45, 50, 55, 60, 65, 70┬░C Saccharomyces cerevisiae

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 80 activity measured after incubating the enzyme in Tris/HCl buffer at various temperatures for 1 h Saccharomyces cerevisiae

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
30 80 activity measured after incubating the enzyme solution for 1 h at various temperatures of 0, 30, 50, 60, 70, 80┬░C in Tris/HCl buffer, activity of 0.222 U/ml at 0┬░C and of 0.238 U/ml at 80┬░C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assayed between pH 5.0 and pH 10.0 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity within, optimum at pH 8.5 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
lower activity above pH 9.0, tested in a range between pH 6-10 Saccharomyces cerevisiae