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Literature summary for 1.1.3.21 extracted from

  • Jacobs, N.J.; VanDemark, P.J.
    The purification and properties of the alpha-glycerophosphate-oxidizing enzyme of Streptococcus faecalis 10C1 (1960), Arch. Biochem. Biophys., 88, 250-255.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Acriflavine 8 mM, 50% inhibition Enterococcus faecalis
Atebrin 25 mM, 50% inhibition Enterococcus faecalis
azide 5 mM, 50% inhibition Enterococcus faecalis
iodoacetate 1 mM, less than 20% inhibition Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
sn-glycerol 3-phosphate
-
Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol-3-phosphate + O2 Enterococcus faecalis
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 Enterococcus faecalis 10C1
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
10C1
-
Enterococcus faecalis 10C1
-
10C1
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
0.118 ml O2/min/mg Enterococcus faecalis

Storage Stability

Storage Stability Organism
2Ā°C, several weeks, slow decline of activity Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + O2
-
Enterococcus faecalis glycerone phosphate + H2O2
-
r
sn-glycerol 3-phosphate + O2
-
Enterococcus faecalis 10C1 glycerone phosphate + H2O2
-
r
sn-glycerol-3-phosphate + O2
-
Enterococcus faecalis glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 2,6-dichloroindophenol, at lower rate than O2 Enterococcus faecalis glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 methylene blue and cytochrome c, at very low rate Enterococcus faecalis glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 high specificity for L-alpha-glycerophosphate Enterococcus faecalis glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2
-
Enterococcus faecalis 10C1 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 2,6-dichloroindophenol, at lower rate than O2 Enterococcus faecalis 10C1 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 methylene blue and cytochrome c, at very low rate Enterococcus faecalis 10C1 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 high specificity for L-alpha-glycerophosphate Enterococcus faecalis 10C1 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Enterococcus faecalis

pH Range

pH Minimum pH Maximum Comment Organism
5 6.7 pH 5.0: about 50% of activity maximum, pH 6.7: about 30% of activity maximum Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group Enterococcus faecalis