Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 40% activation at 1 mM | Gram-negative soil bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-lactate | at concentrations higher than 10 mM | Gram-negative soil bacterium | |
FeSO4 | 86% inhibition at 1 mM | Gram-negative soil bacterium | |
HgCl2 | 28% inhibition at 1 mM | Gram-negative soil bacterium | |
iodoacetate | 33% inhibition at 1 mM | Gram-negative soil bacterium | |
L-leucine | 29% inhibition at 1 mM | Gram-negative soil bacterium | |
L-phenylalanine | 28% inhibition at 1 mM | Gram-negative soil bacterium | |
Lactate | at concentrations higher than 20 mM | Gram-negative soil bacterium | |
MnSO4 | 47% inhibition at 1 mM | Gram-negative soil bacterium | |
p-chloromercuribenzoate | 21% inhibition at 1 mM | Gram-negative soil bacterium | |
ZnSO4 | 28% inhibition at 1 mM | Gram-negative soil bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
(R)-lactate | - |
Gram-negative soil bacterium | |
0.2 | - |
Lactate | - |
Gram-negative soil bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
? * 45000, SDS-PAGE | Gram-negative soil bacterium |
204000 | - |
gel filtration | Gram-negative soil bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gram-negative soil bacterium | - |
- |
- |
Gram-negative soil bacterium KY6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gram-negative soil bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | ? | - |
? | |
(R)-lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium KY6 | ? | - |
? | |
(S)-lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | pyruvate + ? | - |
? | |
(S)-lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium KY6 | pyruvate + ? | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | ? | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium KY6 | ? | - |
? | |
L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | ? | - |
? | |
L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium KY6 | ? | - |
? | |
L-malic acid + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | ? | - |
? | |
lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium | ? | - |
? | |
lactate + 2,6-dichlorophenolindophenol | no decarboxylation | Gram-negative soil bacterium KY6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 45000, SDS-PAGE | Gram-negative soil bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Gram-negative soil bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
45 | stable for 10 min | Gram-negative soil bacterium |
55 | - |
50% activity after 10 min | Gram-negative soil bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Gram-negative soil bacterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | 9.5 | stable for 24 h at 4°C | Gram-negative soil bacterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Gram-negative soil bacterium |