BRENDA - Enzyme Database
show all sequences of 1.1.3.18

Purification and some properties of polyvinyl alcohol-degrading enzyme produced by Pseudomonas O-3

Suzuki, T.; Agric. Biol. Chem. 40, 497-504 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2,4-dinitrophenol
activity 101.6%
Pseudomonas sp.
alpha,alpha'-dipyridyl
activity 102%
Pseudomonas sp.
Monoiodoacetic acid
activity 102.5%
Pseudomonas sp.
Na3AsO3
activity 101.4%
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
-
Pseudomonas sp.
EDTA
-
Pseudomonas sp.
hydroxylamine
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
salicylaldoxine
-
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
cofactor
Pseudomonas sp.
Fe2+
cofactor
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
O-3
-
Pseudomonas sp. O-3
-
O-3
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.73
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
polyvinyl alcohol + O2
-
348174
Pseudomonas sp.
polyvinylketone + H2O2
-
-
-
?
polyvinyl alcohol + O2
-
348174
Pseudomonas sp. O-3
polyvinylketone + H2O2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas sp.
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to, activity completely lost at 75°C
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
11
stable in this range at 5°C, above pH 11 activity decreases rapidly
Pseudomonas sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2,4-dinitrophenol
activity 101.6%
Pseudomonas sp.
alpha,alpha'-dipyridyl
activity 102%
Pseudomonas sp.
Monoiodoacetic acid
activity 102.5%
Pseudomonas sp.
Na3AsO3
activity 101.4%
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
-
Pseudomonas sp.
EDTA
-
Pseudomonas sp.
hydroxylamine
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
salicylaldoxine
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
cofactor
Pseudomonas sp.
Fe2+
cofactor
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.73
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
polyvinyl alcohol + O2
-
348174
Pseudomonas sp.
polyvinylketone + H2O2
-
-
-
?
polyvinyl alcohol + O2
-
348174
Pseudomonas sp. O-3
polyvinylketone + H2O2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
50
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to, activity completely lost at 75°C
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
11
stable in this range at 5°C, above pH 11 activity decreases rapidly
Pseudomonas sp.
Other publictions for EC 1.1.3.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724083
Goswami
An overview on alcohol oxidase ...
Aspergillus terreus
Appl. Microbiol. Biotechnol.
97
4259-4275
2013
-
-
-
-
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-
10
-
1
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6
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5
1
1
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1
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10
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1
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5
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
724152
Veteikyte
-
Secondary alcohol oxidase acti ...
Pseudomonas putida, Pseudomonas putida 4
Biocatal. Agricult. Biotechnol.
2
89-95
2013
-
-
-
-
-
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1
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4
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2
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1
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10
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1
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1
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1
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4
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1
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10
-
1
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1
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1
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1
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-
684619
Vaclavkova
Novel aspects of symbiotic (po ...
Sphingomonas sp.
Appl. Microbiol. Biotechnol.
76
911-917
2007
1
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1
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1
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1
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1
-
2
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-
-
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-
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-
657407
Qian
-
Isolation and culture characte ...
Penicillium sp., Penicillium sp. WSH02-21
World J. Microbiol. Biotechnol.
20
587-591
2004
-
-
-
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1
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2
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3
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1
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1
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1
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4
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287626
Kawagoshi
-
Purification and properties of ...
Brevundimonas vesicularis, Pseudomonas sp., Pseudomonas sp. O-3
World J. Microbiol. Biotechnol.
13
273-277
1997
-
-
-
-
-
-
9
-
2
4
2
-
-
8
-
-
2
-
-
-
1
-
44
2
2
-
1
-
1
-
1
-
-
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-
9
-
-
2
4
2
-
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-
2
-
-
1
-
44
2
2
-
1
-
1
-
1
-
-
-
-
-
-
-
210617
Sakai
-
Degradation mechanism of poly( ...
Pseudomonas sp., Pseudomonas sp. O-3
Agric. Biol. Chem.
50
989-996
1986
-
-
-
-
-
-
-
-
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-
-
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7
-
-
1
-
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10
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1
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10
-
-
-
-
-
-
-
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-
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-
-
-
287623
Shimao
Localization of polyvinyl alco ...
no activity in Pseudomonas putida, no activity in Pseudomonas putida VM15A, Pseudomonas sp., Pseudomonas sp. O-3, Pseudomonas sp. VM15C
Appl. Environ. Microbiol.
49
8-10
1985
-
-
-
-
-
-
-
-
4
-
-
-
-
23
-
-
1
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4
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1
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-
348181
Sakai
-
Purification and properties of ...
Bacteria
Agric. Biol. Chem.
49
817-825
1985
4
-
-
-
-
-
1
-
-
1
4
-
-
1
-
-
1
-
-
-
1
-
48
1
1
-
1
-
1
-
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
1
4
-
-
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-
1
-
-
1
-
48
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
348179
Sakai
-
Separation of secondary alcoho ...
Bacteria
Agric. Biol. Chem.
47
153-155
1983
-
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-
-
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-
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348180
Shimao
-
Purification of membrane-bound ...
no activity in Pseudomonas putida, no activity in Pseudomonas putida VM15A, Pseudomonas sp., Pseudomonas sp. VM15C
FEMS Microbiol. Lett.
20
429-433
1983
-
-
-
-
-
-
5
2
4
-
-
-
-
14
-
-
1
-
-
-
1
-
16
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
4
-
-
-
-
-
-
1
-
-
1
-
16
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
348178
Shimao
Production of polyvinyl alcoho ...
no activity in Pseudomonas putida, no activity in Pseudomonas putida VM15A, Pseudomonas sp., Pseudomonas sp. O-3, Pseudomonas sp. VM15C
Appl. Environ. Microbiol.
44
28-32
1982
-
-
-
-
-
-
-
-
3
-
-
-
-
23
-
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3
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-
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348177
Morita
-
Purification and properties of ...
Pseudomonas boreopolis, Pseudomonas sp.
Agric. Biol. Chem.
43
1225-1235
1979
-
-
-
-
-
-
6
-
-
3
3
-
-
2
-
-
1
-
-
-
1
-
38
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
3
3
-
-
-
-
1
-
-
1
-
38
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
348176
Suzuki
-
Oxidation of secondary alcohol ...
Pseudomonas sp., Pseudomonas sp. O-3
Agric. Biol. Chem.
42
1187-1194
1978
-
-
-
-
-
-
5
-
1
1
1
-
-
7
-
-
1
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
1
1
-
-
-
-
1
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348175
Morita
-
A secondary alcohol oxidase: a ...
Pseudomonas sp.
Agric. Biol. Chem.
41
1535-1537
1977
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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30
-
1
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1
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1
-
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-
-
30
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
348174
Suzuki
-
Purification and some properti ...
Pseudomonas sp., Pseudomonas sp. O-3
Agric. Biol. Chem.
40
497-504
1976
4
-
-
-
-
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5
-
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2
1
-
-
7
-
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1
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1
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2
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1
1
1
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1
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1
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4
-
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5
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2
1
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1
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1
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2
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1
1
1
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1
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1
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