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Literature summary for 1.1.3.17 extracted from

  • Heath, R.S.; Birmingham, W.R.; Thompson, M.P.; Taglieber, A.; Daviet, L.; Turner, N.J.
    An engineered alcohol oxidase for the oxidation of primary alcohols (2019), ChemBioChem, 20, 276-281 .
    View publication on PubMed
Search for more literature for 1.1.3.17

Protein Variants

Protein Variants Comment Organism
M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A/D250G/F253R/V355T/F357R/M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13, with a 20fold increased kcat compared to that of the wildtype enzyme. This variant enables the oxidation of 10 mM hexanol to hexanal in less than 24h with 100% conversion and catalyzes significantly improved oxidation of saturated, unsaturated, aliphatic, cyclic and benzylic alcohols Arthrobacter globiformis
S101A/V355T/F357R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
S101A/V355T/F357R/M359R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis
V355T/F357R mutant displays increased activity with hexan-1-ol, reaction of EC 1.1.3.13 Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 choline wild-type, 30°C, pH 8 Arthrobacter globiformis
1.8
-
 choline mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
2.01
-
 choline mutant M359R, 30°C, pH 8 Arthrobacter globiformis
3.3
-
 choline mutant S101A, 30°C, pH 8 Arthrobacter globiformis
3.4
-
 choline mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
4.7
-
 choline mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
5.8
-
 choline mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.034
-
 wild-type, substrate triethanolamine, 30°C, pH 8 Arthrobacter globiformis
0.09
-
 mutant S101A/D250G/F253R/V355T/F357R/M359R, substrate choline, 30°C, pH 8 Arthrobacter globiformis
10.6
-
 wild-type, substrate choline, 30°C, pH 8 Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + O2 + H2O
-
 Arthrobacter globiformis betaine + 2 H2O2
-
 ?
additional information poor substrate for wild-type, but substrate for mutants is 1-hexanol, reaction of EC 1.1.3.13 Arthrobacter globiformis ?
-
-
triethanolamine + O2
-
 Arthrobacter globiformis ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 wild-type, stable for at least 3 h Arthrobacter globiformis
32
-
 melting temperature of wild-type Arthrobacter globiformis
40
-
 mutant S101A/V355T/F357R/M359R, 3 h, 10% residual activity. Mutant S101A/D250G/F253R/V355T/F357R/M359R, 3 h, almost 50% residual activity Arthrobacter globiformis
52
-
 metling temperature of mutant S101A/D250G/F253R/V355T/F357R/M359R Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 choline mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.02
-
 choline mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.17
-
 choline mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.19
-
 choline mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
4.52
-
 choline mutant S101A, 30°C, pH 8 Arthrobacter globiformis
9.02
-
 choline mutant M359R, 30°C, pH 8 Arthrobacter globiformis
14.5
-
 choline wild-type, 30°C, pH 8 Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 choline mutant S101A/D250G/F253R/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.008
-
 choline mutant S101A/V355T/F357R/M359R, 30°C, pH 8 Arthrobacter globiformis
0.03
-
 choline mutant V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
0.05
-
 choline mutant S101A/V355T/F357R, 30°C, pH 8 Arthrobacter globiformis
1.37
-
 choline mutant S101A, 30°C, pH 8 Arthrobacter globiformis
4.48
-
 choline mutant M359R, 30°C, pH 8 Arthrobacter globiformis
23.8
-
 choline wild-type, 30°C, pH 8 Arthrobacter globiformis