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Literature summary for 1.1.3.17 extracted from

  • Gadda, G.; Yuan, H.
    Substitutions of S101 decrease proton and hydride transfers in the oxidation of betaine aldehyde by choline oxidase (2017), Arch. Biochem. Biophys., 634, 76-82 .
    View publication on PubMed
Search for more literature for 1.1.3.17

Protein Variants

Protein Variants Comment Organism
S101A stopped-flow traces for the reductive half-reaction are monophasic like wild-type choline oxidase Arthrobacter globiformis
S101C contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 Arthrobacter globiformis
S101T contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 Arthrobacter globiformis
S101V contrary to wild-type, stopped-flow traces for the reductive half-reaction are biphasic, corresponding to the reactions of proton abstraction and hydride transfer. The rate constants for proton transfer in the S101T/C/V variants decrease logarithmically with increasing hydrophobicity of residue 101 Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 O2 mutant S101V, pH 10, 25°C Arthrobacter globiformis
0.014
-
 O2 mutant S101C, pH 10, 25°C Arthrobacter globiformis
0.11
-
 Betaine aldehyde mutant S101C, pH 10, 25°C Arthrobacter globiformis
0.21
-
 O2 mutant S101A, pH 10, 25°C Arthrobacter globiformis
0.21
-
 Betaine aldehyde mutant S101T, pH 10, 25°C Arthrobacter globiformis
0.48
-
 O2 mutant S101T, pH 10, 25°C Arthrobacter globiformis
0.54
-
 Betaine aldehyde mutant S101V, pH 10, 25°C Arthrobacter globiformis
2
-
 O2 wild-type, pH 10, 25°C Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + O2 + H2O
-
 Arthrobacter globiformis betaine + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
codA
-
 Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 Betaine aldehyde mutant S101V, pH 10, 25°C Arthrobacter globiformis
0.7
-
 Betaine aldehyde mutant S101C, pH 10, 25°C Arthrobacter globiformis
21
-
 Betaine aldehyde mutant S101T, pH 10, 25°C Arthrobacter globiformis
42
-
 Betaine aldehyde mutant S101A, pH 10, 25°C Arthrobacter globiformis
133
-
 Betaine aldehyde wild-type, pH 10, 25°C Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
 Betaine aldehyde mutant S101V, pH 10, 25°C Arthrobacter globiformis
6.1
-
 Betaine aldehyde mutant S101C, pH 10, 25°C Arthrobacter globiformis
32
-
 O2 mutant S101V, pH 10, 25°C Arthrobacter globiformis
43
-
 O2 mutant S101T, pH 10, 25°C Arthrobacter globiformis
48
-
 O2 mutant S101C, pH 10, 25°C Arthrobacter globiformis
53
-
 O2 wild-type, pH 10, 25°C Arthrobacter globiformis
99
-
 Betaine aldehyde mutant S101T, pH 10, 25°C Arthrobacter globiformis
203
-
 O2 mutant S101A, pH 10, 25°C Arthrobacter globiformis