Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dithionite | reduction of FMN | Rattus sp. | |
L-leucine | competitive inhibition | Rattus sp. | |
pyruvate | competitive inhibition | Rattus sp. | |
sodium sulfite | reduces FMN | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
L-alpha-hydroxyphenyllactate | - |
Rattus sp. | |
3.2 | - |
DL-2-hydroxycaproate | - |
Rattus sp. | |
8 | - |
DL-2-hydroxyisovalerate | - |
Rattus sp. | |
13 | - |
DL-2-hydroxyvalerate | - |
Rattus sp. | |
14 | - |
DL-2-hydroxybutyrate | - |
Rattus sp. | |
15 | - |
L-leucine | - |
Rattus sp. | |
27 | - |
DL-lactate | - |
Rattus sp. | |
28 | - |
DL-3-chlorolactate | - |
Rattus sp. | |
40 | - |
L-tryptophan | - |
Rattus sp. | |
53 | - |
L-methionine | - |
Rattus sp. | |
71 | - |
DL-alpha-phenyllactate | - |
Rattus sp. | |
90 | - |
L-lysine | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
4 * 47500, SDS-PAGE | Rattus sp. |
190000 | - |
gel filtration | Rattus sp. |
250000 | - |
sedimentation equilibrium analysis | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | Rattus sp. | - |
2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | Rattus sp. | - |
2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | Rattus sp. | low activity | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | Rattus sp. | - |
2-oxovalerate + H2O2 | - |
? | |
DL-lactate + O2 | Rattus sp. | - |
pyruvate + H2O2 | - |
? | |
glycolate + O2 | Rattus sp. | preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase | glyoxylate + H2O2 | - |
? | |
L-leucine + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-lysine + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-methionine + O2 | Rattus sp. | - |
? + H2O2 | - |
? | |
L-tryptophan + O2 | Rattus sp. | - |
? + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | - |
Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | - |
Rattus sp. | 2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | low activity | Rattus sp. | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | - |
Rattus sp. | 2-oxovalerate + H2O2 | - |
? | |
DL-3-chlorolactate + O2 | best substrate tested | Rattus sp. | 3-chloropyruvate + H2O2 | - |
? | |
DL-alpha-phenyllactate + O2 | - |
Rattus sp. | ? + H2O2 | - |
r | |
DL-lactate + O2 | - |
Rattus sp. | pyruvate + H2O2 | - |
? | |
DL-phenyllactate + O2 | - |
Rattus sp. | phenylpyruvate + H2O2 | - |
? | |
glycolate + O2 | preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase | Rattus sp. | glyoxylate + H2O2 | - |
? | |
L-2-hydroxyphenyllactate + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-alpha-hydroxyphenyllactate + O2 | - |
Rattus sp. | ? + H2O2 | - |
r | |
L-leucine + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-lysine + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-methionine + O2 | - |
Rattus sp. | ? + H2O2 | - |
? | |
L-tryptophan + O2 | - |
Rattus sp. | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 47500, SDS-PAGE | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Rattus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
half-maximum oxidation rate | Rattus sp. |
9 | - |
half-maximum oxidation rate | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one FAD per two subunits | Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
L-leucine | - |
Rattus sp. |