General Stability | Organism |
---|---|
loss of activity due to loss of irreversibly removed FMN during storage | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, isoenzymes A and B, SDS-PAGE | Rattus sp. |
40000 | - |
x * 40000, isoenzymes A and B, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | Mus musculus | substrate for isoenzyme A | glyoxylate + H2O2 | - |
? | |
glycolate + O2 | Rattus sp. | substrate for isoenzyme A | glyoxylate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | Mus musculus | substrate for isoenzyme B | 2-oxoisocaproate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | Rattus sp. | substrate for isoenzyme B | 2-oxoisocaproate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, isoenzymes A and B | Mus musculus |
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
kidney | isoenzyme L-2-hydroxyacid oxidase B | Rattus sp. | - |
liver | - |
Mus musculus | - |
liver | isoenzyme L-2-hydroxyacid oxidase A | Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
isoenzyme B | Rattus sp. |
3.66 | - |
isoemzyme A | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + O2 | substrate for isoenzyme A | Mus musculus | glyoxylate + H2O2 | - |
? | |
glycolate + O2 | substrate for isoenzyme A | Rattus sp. | glyoxylate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | substrate for isoenzyme B | Mus musculus | 2-oxoisocaproate + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | substrate for isoenzyme B | Rattus sp. | 2-oxoisocaproate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, isoenzymes A and B, SDS-PAGE | Rattus sp. |
? | x * 40000, isoenzymes A and B, SDS-PAGE | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Mus musculus | |
FMN | one FAD per subunit | Rattus sp. |