Literature summary for 1.1.3.15 extracted from

Payne, M.S.; Petrillo, K.L.; Gavagan, J.E.; Wagner, L.W.; DiCosimo, R.; Anton, D.L.
High-level production of spinach glycolate oxidase in the methylotrophic yeast Pichia pastoris: engineering a biocatalyst (1995), Gene, 167, 215-219.

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Application

Application	Comment	Organism
synthesis	-	Spinacia oleracea

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Spinacia oleracea

Crystallization (Commentary)

Crystallization (Comment)	Organism
ammonium sulfate precipitation, 2.6 A resolution of a two subunit apoenzyme	Spinacia oleracea

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Spinacia oleracea	5777	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glycolate + O2	Spinacia oleracea	-	glyoxylate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Spinacia oleracea	-	spinach	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Spinacia oleracea

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Spinacia oleracea	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycolate + O2	-	Spinacia oleracea	glyoxylate + H2O2	-	?

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Spinacia oleracea

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Release 2023.1 (January 2023)