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Literature summary for 1.1.3.13 extracted from

  • Mangkorn, N.; Kanokratana, P.; Roongsawang, N.; Laosiripojana, N.; Champreda, V.
    Purification, characterization, and stabilization of alcohol oxidase from Ogataea thermomethanolica (2018), Protein Expr. Purif., 150, 26-32 .
    View publication on PubMed
Search for more literature for 1.1.3.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 methanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
5.41
-
 ethanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
24.91
-
 1-propanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)

Organism

Organism UniProt Comment Textmining
Ogataea thermomethanolica (nom. inval.) A0A067YIW8
-
-

Purification (Commentary)

Purification (Comment) Organism
single step chromatographic separation on a DEAE-Sepharose column Ogataea thermomethanolica (nom. inval.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.34
-
 pH 7.5, 30°C, substrate methanol Ogataea thermomethanolica (nom. inval.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2
-
 Ogataea thermomethanolica (nom. inval.) butanal + H2O2
-
 ?
1-pentanol + O2
-
 Ogataea thermomethanolica (nom. inval.) pentanal + H2O2
-
 ?
1-propanol + O2
-
 Ogataea thermomethanolica (nom. inval.) propanal + H2O2
-
 ?
ethanol + O2
-
 Ogataea thermomethanolica (nom. inval.) acetaldehyde + H2O2
-
 ?
methanol + O2
-
 Ogataea thermomethanolica (nom. inval.) formaldehyde + H2O2
-
 ?
additional information very poor substrates: 1-hexanol, 1-octanol, 2-propanol, t-butanol, 3-pentanol, 3-methyl-1-butanol, glycerol Ogataea thermomethanolica (nom. inval.) ?
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Ogataea thermomethanolica (nom. inval.)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 presence of alginic acid (0.35%) enhances thermal stability, resulting in 72% increase in its half-life at 40°C under the operational conditions Ogataea thermomethanolica (nom. inval.)
40
-
 6 h, 40% residual activity Ogataea thermomethanolica (nom. inval.)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60.48
-
 methanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
93.83
-
 1-propanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
103.9
-
 ethanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Ogataea thermomethanolica (nom. inval.)

pH Range

pH Minimum pH Maximum Comment Organism
6 8 more than 60% of maximum activity Ogataea thermomethanolica (nom. inval.)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8
-
 1-propanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
19.2
-
 ethanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)
227.3
-
 methanol pH 7.5, 30°C Ogataea thermomethanolica (nom. inval.)