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Literature summary for 1.1.3.13 extracted from

  • Nguyen, Q.T.; Romero, E.; Dijkman, W.P.; de Vasconcellos, S.P.; Binda, C.; Mattevi, A.; Fraaije, M.W.
    Structure-based engineering of Phanerochaete chrysosporium alcohol oxidase for enhanced oxidative power toward glycerol (2018), Biochemistry, 57, 6209-6218 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
structure to 2.6 A resolution. The catalytic center is a solvent-inaccessible cavity located at the re side of the flavin cofactor. Its small size explains the observed preference for methanol and ethanol as best substrates Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
F101N with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol Phanerodontia chrysosporium
F101S with enlarged catalytic cavity, retains a high degree of thermostability Phanerodontia chrysosporium
M103S with enlarged catalytic cavity, increase in activity with substrates 1-propanol, glycerol, (R)-1,2-propanediol Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
1
-
 ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
2
-
 methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
2
-
 methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
2
-
 methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
2
-
 1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
4
-
 1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
11
-
 ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
11
-
 1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
14
-
 methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
15
-
 ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
45
-
 1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
110
-
 (R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
110
-
 (R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1200
-
 (R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1600
-
 (R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium T2M2J4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1,2-propanediol + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1-propanol + O2
-
 Phanerodontia chrysosporium propanal + H2O2
-
 ?
ethanol + O2
-
 Phanerodontia chrysosporium acetaldehyde + H2O2
-
 ?
methanol + O2
-
 Phanerodontia chrysosporium formaldehyde + H2O2
-
 ?
additional information poor substrate: glycerol Phanerodontia chrysosporium ?
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
 melting temperature of mutant F101S, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium
52
-
 melting temperature of mutant F101N, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium
58
-
 melting temperature of wild-type, 5 mM phoshate buffer pH 7.5 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 (R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1
-
 ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1
-
 methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1
-
 1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
4
-
 methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
4
-
 (R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
5
-
 (R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
5
-
 ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
7
-
 1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
10
-
 ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
10
-
 methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
11
-
 1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
12
-
 1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
13
-
 (R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
18
-
 methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
19
-
 ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
7 10 more than 75% of maximum activity Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Phanerodontia chrysosporium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
 (R)-1,2-Propanediol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
0.004
-
 (R)-1,2-Propanediol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
0.011
-
 (R)-1,2-Propanediol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
0.036
-
 (R)-1,2-Propanediol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
0.24
-
 1-propanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
0.5
-
 methanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
0.5
-
 1-propanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
0.71
-
 methanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
0.91
-
 ethanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1
-
 ethanol mutant M103S, pH 7.5, 23°C Phanerodontia chrysosporium
1.1
-
 1-propanol mutant F101N, pH 7.5, 23°C Phanerodontia chrysosporium
1.3
-
 ethanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium
1.8
-
 1-propanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
2
-
 methanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
5
-
 ethanol mutant F101S, pH 7.5, 23°C Phanerodontia chrysosporium
9
-
 methanol wild-type, pH 7.5, 23°C Phanerodontia chrysosporium