Cloned (Comment) | Organism |
---|---|
functional recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 from the plasmid pPIC9K-AOX intracellularly and extracellularly, batch culture. Expression in Escherichia coli strain DH5alpha in batch culture. Method comparison and optimization, overview | Komagataella pastoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits at 5 mM, at 7.8 mM the enzyme maintains 50% of its initial activity after 0.5 h of incubation at 4°C | Komagataella pastoris |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
hexanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits slightly after 8 h | Komagataella pastoris |
isoamyl alcohol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
isobutanol | pH 8.0, only slight effect on enzyme activity at 30% v/v | Komagataella pastoris |
isopropanol | pH 8.0, activates at 30% v/v for 3 h, then inhibits strongly up after h | Komagataella pastoris |
Methanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
additional information | effects of various solvents on the stability and activity of recmbinant AOX, overview | Komagataella pastoris |
n-Butanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris GS115 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography and gel filtration. The recombinant enzyme shows high thermal stability and resistance to H2O2 inhibition | Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.85 | - |
purified recombinant intracellular enzyme, pH 80, 25°C | Komagataella pastoris |
12.94 | - |
purified recombinant extracellular enzyme, pH 80, 25°C | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + O2 | - |
Komagataella pastoris | ? + H2O2 | - |
? | |
1,3-butylene glycol + O2 | - |
Komagataella pastoris | ? + H2O2 | - |
? | |
1,3-butylene glycol + O2 | - |
Komagataella pastoris GS115 | ? + H2O2 | - |
? | |
2-propanol + O2 | - |
Komagataella pastoris | 2-oxopropane + H2O2 | - |
? | |
ethanol + O2 | - |
Komagataella pastoris | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | - |
Komagataella pastoris GS115 | acetaldehyde + H2O2 | - |
? | |
hexanol + O2 | - |
Komagataella pastoris | hexanal + H2O2 | - |
? | |
hexanol + O2 | - |
Komagataella pastoris GS115 | hexanal + H2O2 | - |
? | |
methanol + O2 | - |
Komagataella pastoris | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Komagataella pastoris GS115 | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | - |
Komagataella pastoris | butanal + H2O2 | - |
? | |
n-butanol + O2 | - |
Komagataella pastoris GS115 | butanal + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AOX | - |
Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Komagataella pastoris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
purified recombinant His-tagged enzyme, pH 8.0, 60 min, stable | Komagataella pastoris |
50 | - |
purified recombinant His-tagged enzyme, pH 8.0, 50 min, 50% activity remaining | Komagataella pastoris |
55 | - |
purified recombinant His-tagged enzyme, pH 8.0, 30 min, inactivation | Komagataella pastoris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
purified recombinant His-tagged enzyme, 25°C, 3 h, inactivation | Komagataella pastoris |
5.5 | 7 | purified recombinant His-tagged enzyme, 25°C, 3 h, 50% activity remaining | Komagataella pastoris |
8 | 10 | purified recombinant His-tagged enzyme, 25°C, 3 h, stable | Komagataella pastoris |