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Literature summary for 1.1.3.13 extracted from

  • Zhang, A.L.; Luo, J.X.; Zhang, T.Y.; Pan, Y.W.; Tan, Y.H.; Fu, C.Y.; Tu, F.Z.
    Recent advances on the GAP promoter derived expression system of Pichia pastoris (2009), Mol. Biol. Rep., 36, 1611-1619.
    View publication on PubMed

Application

Application Comment Organism
molecular biology Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins possessing a strong and tightly regulated promoter from the alcohol oxidase I, AOX1, gene. The transformed cells need to be activated by methanol and grow on methanol as carbon source. With the inducible AOX1 promoter an increase of the copy number above two resulted in a decrease of expression. Combined use of GAP and AOX1 promoters in Pichia pastoris, overview Komagataella pastoris

Cloned(Commentary)

Cloned (Comment) Organism
gene AOX1, strong and tightly regulated promoter inducible by methanol Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
gene AOX1
-

Synonyms

Synonyms Comment Organism
alcohol oxidase I
-
Komagataella pastoris
AOXI
-
Komagataella pastoris