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Literature summary for 1.1.3.13 extracted from

  • Kumar, A.K.; Goswami, P.
    Dissociation and reconstitution studies of a broad substrate specific multimeric alcohol oxidase protein produced by Aspergillus terreus (2009), J. Biochem., 145, 259-265.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DMSO complete inhibition by 50% DMSO Aspergillus terreus
additional information no inhibition by ethylene glycol, even at high concentrations Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Aspergillus terreus 16020
-
microsome
-
Aspergillus terreus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
269000
-
native enzyme, gel filtration Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus MTCC 6324
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by microsomal membrane preparation by differential centrifugation. The enzyme is solubilized from the microsomal membrane using 0.5% (v/v) 3-[(3-cholamidopropyl) dimethyl ammonio] propane-1-sulfonic acid, i.e. CHAPS, and further purified by ammonium sulfate fractionation followed by anion exchange chromatography Aspergillus terreus

Renatured (Commentary)

Renatured (Comment) Organism
enzyme dissociation by 2 M KBr in 0.25 M potassium phosphate, pH 7.5, 0.3 mM EDTA, 5 mM 2-mercaptoethanol and 20% glycerol, for 4 days at 4°C. The inactive dissociated enzyme protein is renaturated, but still catalytically inactive, by assembly after removal of 2-mercaptoethanol and incubation with cofactor FAD. The enzyme activity is regained by incubation with the substrate. The dissociation process traverses through two FAD-associated intermediate proteins, one of whhich shows the enzyme activity. The catalytic reactivation is a slow process. Dynamic light scattering analysis, overview. DMSO and ethylene glycol are ineffective in dissociating this alcohol oxidase Aspergillus terreus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity of native, intermediate dissociated protein, and re-associated protein, overview. The re-associated enzyme protein shows poor activity with substrate (R)-2-octanol Aspergillus terreus
37
-
native intermediate dissociated enzyme protein, substrate (R)-2-octanol Aspergillus terreus
42
-
re-associated enzyme protein, substrate n-dodecanol Aspergillus terreus
45
-
re-associated enzyme protein, substrate phenyl-3-propanol Aspergillus terreus
49
-
native intermediate dissociated enzyme protein, substrate n-dodecanol Aspergillus terreus
62
-
native enzyme, substrate n-dodecanol Aspergillus terreus
63
-
native enzyme, substrate (R)-2-octanol Aspergillus terreus
96
-
re-associated enzyme protein, substrate n-heptanol Aspergillus terreus
183
-
native intermediate dissociated enzyme protein, substrate phenyl-3-propanol Aspergillus terreus
190
-
native intermediate dissociated enzyme protein, substrate n-heptanol Aspergillus terreus
250
-
native enzyme, substrate phenyl-3-propanol Aspergillus terreus
305
-
native enzyme, substrate n-heptanol Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-octanol + O2
-
Aspergillus terreus octan-2-one + H2O2
-
?
(R)-2-octanol + O2
-
Aspergillus terreus MTCC 6324 octan-2-one + H2O2
-
?
3-phenylpropan-1-ol + O2
-
Aspergillus terreus 3-phenylpropanal + H2O2
-
?
3-phenylpropan-1-ol + O2
-
Aspergillus terreus MTCC 6324 3-phenylpropanal + H2O2
-
?
additional information substrate specificity of native, intermediate dissociated protein and re-associated protein, overview Aspergillus terreus ?
-
?
additional information substrate specificity of native, intermediate dissociated protein and re-associated protein, overview Aspergillus terreus MTCC 6324 ?
-
?
n-dodecanol + O2
-
Aspergillus terreus n-dodecanal + H2O2
-
?
n-dodecanol + O2
-
Aspergillus terreus MTCC 6324 n-dodecanal + H2O2
-
?
n-heptanol + O2
-
Aspergillus terreus n-heptanal + H2O2
-
?
n-heptanol + O2
-
Aspergillus terreus MTCC 6324 n-heptanal + H2O2
-
?

Subunits

Subunits Comment Organism
multimer the enzyme is composed of several subunits of of 85, 63, 43, 27 and 13 kDa, the 27 kDa subunit is degraded to 25 kDa, overview Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
FAD essential Aspergillus terreus