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Literature summary for 1.1.3.10 extracted from

  • Abrera, A.; Chang, H.; Kracher, D.; Ludwig, R.; Haltrich, D.
    Characterization of pyranose oxidase variants for bioelectrocatalytic applications (2020), Biochim. Biophys. Acta, 1868, 140335 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trametes ochracea

Protein Variants

Protein Variants Comment Organism
L545C pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency. L545C performs best among the variants studied Trametes ochracea
L547R pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea
Q448H pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea
T166R pre-steady-state kinetics of the oxidative half-reaction show higher kinetic constants as compared to the wild-type. The variant immobilized on glassy carbon electrodes displays high catalytic efficiency Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 1,4-benzoquinone
-
Trametes ochracea 2-dehydro-D-glucose + hydroquinone
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Trametes ochracea 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + ferricenium ion
-
Trametes ochracea 2-dehydro-D-glucose + ferrocenium ion
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Trametes ochracea

Synonyms

Synonyms Comment Organism
P2O
-
Trametes ochracea