Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star DE3 cells | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
L545C | the mutant shows 19% of wild type activity | Trametes ochracea |
L547R | the mutant shows 0.99% of wild type activity | Trametes ochracea |
N593C | the mutant shows 0.058% of wild type activity | Trametes ochracea |
Q448H | the mutant shows 1.5% of wild type activity | Trametes ochracea |
T166R | the mutant shows 39% of wild type activity | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
D-glucose | mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.042 | - |
D-glucose | mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.046 | - |
D-glucose | mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.057 | - |
D-glucose | mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.082 | - |
D-glucose | mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.092 | - |
D-glucose | mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.12 | - |
D-glucose | mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.13 | - |
D-glucose | wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.14 | - |
D-glucose | mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.14 | - |
D-glucose | mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.18 | - |
D-glucose | mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.23 | - |
D-glucose | mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.24 | - |
D-glucose | mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.24 | - |
D-glucose | wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.25 | - |
D-glucose | wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.4 | - |
D-glucose | mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.56 | - |
D-glucose | mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.72 | - |
D-glucose | mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.81 | - |
D-glucose | mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.98 | - |
D-glucose | mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1.6 | - |
D-glucose | mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1.7 | - |
D-glucose | mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1.9 | - |
D-glucose | wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
2.1 | - |
D-glucose | mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | - |
- |
Purification (Comment) | Organism |
---|---|
IMAC Ni-charged resin column chromatography | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Trametes ochracea | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + ferricenium ion | - |
Trametes ochracea | 2-dehydro-D-glucose + ferrocenium ion | - |
? | |
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
glucose 2-oxidase | - |
Trametes ochracea |
POX | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-glucose | mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
0.38 | - |
D-glucose | mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1.1 | - |
D-glucose | mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
2.7 | - |
D-glucose | mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
3.9 | - |
D-glucose | mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
4.6 | - |
D-glucose | mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
8.1 | - |
D-glucose | mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
11 | - |
D-glucose | mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
12 | - |
D-glucose | mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
22 | - |
D-glucose | mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
26 | - |
D-glucose | mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
27 | - |
D-glucose | mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
42 | - |
D-glucose | wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
50 | - |
D-glucose | mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
70 | - |
D-glucose | wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
110 | - |
D-glucose | mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
134 | - |
D-glucose | mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
134 | - |
D-glucose | mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
142 | - |
D-glucose | mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
151 | - |
D-glucose | wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
152 | - |
D-glucose | wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
166 | - |
D-glucose | mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
265 | - |
D-glucose | mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
442 | - |
D-glucose | mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-glucose | mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
2.8 | - |
D-glucose | mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
4.6 | - |
D-glucose | mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
22 | - |
D-glucose | mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
27 | - |
D-glucose | mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
32 | - |
D-glucose | mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
34 | - |
D-glucose | mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
38 | - |
D-glucose | wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
48 | - |
D-glucose | mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
58 | - |
D-glucose | mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
70 | - |
D-glucose | mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
79 | - |
D-glucose | mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
120 | - |
D-glucose | mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
127 | - |
D-glucose | mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
203 | - |
D-glucose | mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
281 | - |
D-glucose | mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
308 | - |
D-glucose | wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
584 | - |
D-glucose | mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
592 | - |
D-glucose | wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
632 | - |
D-glucose | wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1193 | - |
D-glucose | mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1213 | - |
D-glucose | mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
1465 | - |
D-glucose | mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea | |
2608 | - |
D-glucose | mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C | Trametes ochracea |