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Literature summary for 1.1.3.10 extracted from

  • Taniguchi, S.; Chosrowjan, H.; Wongnate, T.; Sucharitakul, J.; Chaiyen, P.; Tanaka, F.
    Ultrafast fluorescence dynamics of flavin adenine dinucleotide in pyranose 2-oxidases variants and their complexes with acetate: conformational heterogeneity with different dielectric constants (2012), J. Photochem. Photobiol. A, 245, 33-42.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Trametes ochracea

Protein Variants

Protein Variants Comment Organism
H167A site-directed mutagenesis, the covalent linkag of the isoalloxazine ring of the cofactor FAD to His167 is destroyed, molecular dynamics, overview Trametes ochracea
H169S site-directed mutagenesis, spectroscopic analysis and molecular dynamics of the T169S P2O-acetate complex Trametes ochracea
additional information comparison the P2O active site in H167A, T169S or T169S-acetate complex with wild-type P2O-acetate complex, molecuar dynamics and spectroscopic analysi, detailed overview Trametes ochracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
-
Trametes ochracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Trametes ochracea
-
2-dehydro-D-glucose + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trametes ochracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recmbinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Trametes ochracea

Synonyms

Synonyms Comment Organism
P2O
-
Trametes ochracea
pyranose 2-Oxidase
-
Trametes ochracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD one FAD per subunit, Trp168 residue near the flavin isoalloxazine ring can potentially act as an electron donor to the excited flavin isoalloxazine ring, isoalloxazine is covalently linked to His167 Trametes ochracea

General Information

General Information Comment Organism
additional information comparison the P2O active site in H167A, T169S or T169S-acetate complex with wild-type P2O-acetate complex, molecuar dynamics and spectroscopic analysi, detailed overview Trametes ochracea