Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.10 extracted from

  • Salaheddin, C.; Takakura, Y.; Tsunashima, M.; Stranzinger, B.; Spadiut, O.; Yamabhai, M.; Peterbauer, C.K.; Haltrich, D.
    Characterisation of recombinant pyranose oxidase from the cultivated mycorrhizal basidiomycete Lyophyllum shimeji (hon-shimeji) (2010), Microb. Cell Fact., 9, 57.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology P2Ox is a biocatalyst with high potential for biotransformations of carbohydrates and in synthetic carbohydrate chemistry. P2Ox is useful as bioelement in biofuel cells, replacing glucose oxidase Lyophyllum shimeji

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Lyophyllum shimeji

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
0.187
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
0.187
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
0.314
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
3.84
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
6.3
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
14.7
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
72.8
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
gel filtration Lyophyllum shimeji
68490
-
calculated from amino acid sequence Lyophyllum shimeji

Organism

Organism UniProt Comment Textmining
Lyophyllum shimeji Q75ZP8
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography and preparative native PAGE Lyophyllum shimeji

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + O2
-
Lyophyllum shimeji 2-dehydro-D-galactose + H2O2
-
?
D-glucose + 1,4-benzoquinone
-
Lyophyllum shimeji 2-dehydro-D-glucose + 1,4-hydroquinone
-
?
D-glucose + 2,6-dichloroindophenol
-
Lyophyllum shimeji 2-dehydro-D-glucose + ?
-
?
D-glucose + ferrocenium ion
-
Lyophyllum shimeji 2-dehydro-D-glucose + ?
-
?
D-glucose + O2
-
Lyophyllum shimeji 2-dehydro-D-glucose + H2O2
-
?
D-xylose + O2
-
Lyophyllum shimeji D-xylosone + H2O2
-
?
L-sorbose + O2
-
Lyophyllum shimeji 5-dehydro-D-fructose + H2O2
-
?
melibiose + O2
-
Lyophyllum shimeji ?
-
?

Synonyms

Synonyms Comment Organism
P2Ox
-
Lyophyllum shimeji
pyranose 2-Oxidase
-
Lyophyllum shimeji
pyranose:oxygen 2-oxidoreductase
-
Lyophyllum shimeji

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 55 the melting temperature is 54.9°C, half-life time of activity is 0.12 min at 50°C and pH 6.5, half-life time of activity is 60 h at 40°C and pH 6.5/8.0 and 43 min at 40°C and pH 4.0 Lyophyllum shimeji

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.839
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji
1.02
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
5.48
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
6.92
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
7.7
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
39.9
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
67.3
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
92.3
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Lyophyllum shimeji

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0115
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji
0.265
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
0.524
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
0.867
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
22.1
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
213
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
361
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
2760
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji