Application | Comment | Organism |
---|---|---|
biotechnology | P2Ox is a biocatalyst with high potential for biotransformations of carbohydrates and in synthetic carbohydrate chemistry. P2Ox is useful as bioelement in biofuel cells, replacing glucose oxidase | Lyophyllum shimeji |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2(DE3) cells | Lyophyllum shimeji |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
1,4-benzoquinone | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.187 | - |
2,6-dichloroindophenol | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.187 | - |
ferrocenium ion | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.314 | - |
D-glucose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
3.84 | - |
D-galactose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
6.3 | - |
D-xylose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
14.7 | - |
L-sorbose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
72.8 | - |
melibiose | at 30°C, pH 6.5 | Lyophyllum shimeji |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Lyophyllum shimeji |
68490 | - |
calculated from amino acid sequence | Lyophyllum shimeji |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lyophyllum shimeji | Q75ZP8 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography and preparative native PAGE | Lyophyllum shimeji |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | - |
Lyophyllum shimeji | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Lyophyllum shimeji | 2-dehydro-D-glucose + 1,4-hydroquinone | - |
? | |
D-glucose + 2,6-dichloroindophenol | - |
Lyophyllum shimeji | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + ferrocenium ion | - |
Lyophyllum shimeji | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + O2 | - |
Lyophyllum shimeji | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-xylose + O2 | - |
Lyophyllum shimeji | D-xylosone + H2O2 | - |
? | |
L-sorbose + O2 | - |
Lyophyllum shimeji | 5-dehydro-D-fructose + H2O2 | - |
? | |
melibiose + O2 | - |
Lyophyllum shimeji | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P2Ox | - |
Lyophyllum shimeji |
pyranose 2-Oxidase | - |
Lyophyllum shimeji |
pyranose:oxygen 2-oxidoreductase | - |
Lyophyllum shimeji |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | the melting temperature is 54.9°C, half-life time of activity is 0.12 min at 50°C and pH 6.5, half-life time of activity is 60 h at 40°C and pH 6.5/8.0 and 43 min at 40°C and pH 4.0 | Lyophyllum shimeji |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.839 | - |
melibiose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
1.02 | - |
D-galactose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
5.48 | - |
D-xylose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
6.92 | - |
D-glucose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
7.7 | - |
L-sorbose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
39.9 | - |
ferrocenium ion | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
67.3 | - |
2,6-dichloroindophenol | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
92.3 | - |
1,4-benzoquinone | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Lyophyllum shimeji |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0115 | - |
melibiose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.265 | - |
D-galactose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.524 | - |
L-sorbose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
0.867 | - |
D-xylose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
22.1 | - |
D-glucose | at 30°C, pH 6.5 | Lyophyllum shimeji | |
213 | - |
ferrocenium ion | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
361 | - |
2,6-dichloroindophenol | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji | |
2760 | - |
1,4-benzoquinone | using D-glucose as cosubstrate, at 30°C, pH 6.5 | Lyophyllum shimeji |