Protein Variants | Comment | Organism |
---|---|---|
T169A | the mutant shows severely decreased catalytic efficiency for D-glucose and D-galactose compared to the wild type enzyme | Trametes ochracea |
T169G | the mutant shows decreased catalytic efficiency for D-glucose and D-galactose compared to the wild type enzyme | Trametes ochracea |
T169N | the mutant shows decreased catalytic efficiency for D-glucose and increased catalytic efficiency for D-galactose compared to the wild type enzyme | Trametes ochracea |
T169S | the mutant shows increased kcat and Km values for D-glucose and D-galactose compared to the wild type enzyme | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
O2 | wild type enzyme, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.07 | - |
O2 | mutant enzyme T169S, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.09 | - |
O2 | wild type enzyme, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.2 | - |
O2 | mutant enzyme T169A, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.4 | - |
O2 | mutant enzyme T169A, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.9 | - |
D-glucose | mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.99 | - |
O2 | mutant enzyme T169S, using D-glucose as cosubstrate ,in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
1.13 | - |
D-glucose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
1.32 | - |
O2 | mutant enzyme T169N, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
1.7 | - |
D-glucose | mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
3.1 | - |
D-galactose | mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
3.7 | - |
O2 | mutant enzyme T169G, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
4.5 | - |
O2 | mutant enzyme T169G, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
4.6 | - |
D-glucose | mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
4.8 | - |
O2 | mutant enzyme T169N, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
15 | - |
D-galactose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
16 | - |
D-galactose | mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
16.2 | - |
D-galactose | mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
30 | - |
D-glucose | mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
50 | - |
D-galactose | mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | - |
Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-glucose | mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.7 | - |
D-galactose | mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
1.2 | - |
D-galactose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
5.6 | - |
D-glucose | mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
9.7 | - |
D-glucose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
13.7 | - |
D-galactose | mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
D-glucose | mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.005 | - |
D-galactose | mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.07 | - |
O2 | mutant enzyme T169A, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.08 | - |
D-galactose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.15 | - |
D-galactose | mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.2 | - |
O2 | mutant enzyme T169G, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.3 | - |
D-galactose | mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.4 | - |
O2 | mutant enzyme T169A, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.4 | - |
O2 | mutant enzyme T169G, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.5 | - |
D-galactose | mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
0.8 | - |
D-glucose | mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
0.99 | - |
O2 | mutant enzyme T169S, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
1.2 | - |
D-glucose | mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
3.4 | - |
O2 | mutant enzyme T169N, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
4.8 | - |
O2 | mutant enzyme T169N, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
8.1 | - |
D-glucose | mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
8.6 | - |
D-glucose | wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea | |
32 | - |
O2 | mutant enzyme T169S, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
44.2 | - |
O2 | wild type enzyme, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C | Trametes ochracea | |
110 | - |
O2 | wild type enzyme, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C | Trametes ochracea |