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Literature summary for 1.1.3.10 extracted from

  • Spadiut, O.; Tan, T.C.; Pisanelli, I.; Haltrich, D.; Divne, C.
    Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase (2010), FEBS J., 277, 2892-2909.
    View publication on PubMed
Search for more literature for 1.1.3.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Trametes ochracea

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2 Trametes ochracea

Protein Variants

Protein Variants Comment Organism
F454A/S455A/Y456A the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
F454A/Y456A the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
F454N the apparent kcat value with D-glucose and O2 (fixed at a concentration of 0.256 mM, air saturation) as substrates decreases dramatically compared to the wild type enzyme Trametes ochracea
F454N the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
F454P the mutant shows strongly decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
H450Q the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme Trametes ochracea
additional information the deletion mutant DELTA454-456 shows 6fold lower kcat (D-galactose/O2), and 0.8fold elevated Km (D-galactose) value compared to the wild type enzyme Trametes ochracea
Y456W the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0071
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0089
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.01
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.013
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.015
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.016
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.023
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.025
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.027
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.029
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.029
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.041
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.049
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.052
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.072
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.072
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.078
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.1
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.14
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.14
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.15
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.24
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.24
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.28
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.35
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.38
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.4
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.76
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.5
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.5
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.7
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.8
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2 3 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.1
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.5
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
6.1
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
10
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
13
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
13
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
26
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
29
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
34
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
50
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
240
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
260
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
350
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
390
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1500
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 gel filtration Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32 synonym Trametes ochracea
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-immobilized metal affinity column chromatography Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-2-fluoro-D-glucose + O2 slow substrate Trametes ochracea 2-deoxy-3-dehydro-D-glucose + H2O2
-
 ?
beta-D-galactose + O2 poor substrate Trametes ochracea 2-dehydro-D-galactose + H2O2
-
 ?
D-galactose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-galactose + hydroquinone
-
 ?
D-galactose + ferrocenium hexafluorophosphate
-
 Trametes ochracea 2-dehydro-D-galactose + ferrocene
-
 ?
D-glucose + 1,4-benzoquinone 1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction Trametes ochracea 2-dehydro-D-glucose + hydroquinone
-
 ?
D-glucose + ferrocenium hexafluorophosphate
-
 Trametes ochracea 2-dehydro-D-glucose + ferrocene
-
 ?
D-glucose + O2 the wild type enzyme displays a clear preference for D-glucose over D-galactose Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?
D-melibiose + O2
-
 Trametes ochracea ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 2 * 000, SDS-PAGE Trametes ochracea

Synonyms

Synonyms Comment Organism
P2O
-
 Trametes ochracea
pyranose 2-Oxidase
-
 Trametes ochracea
pyranose:oxygen 2-oxidoreductase
-
 Trametes ochracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the half life of the wild type enzyme at 60°C and 70°C is 12 min and 0.07 min, respectively. The melting temperature of the wild type enzyme is at 63.5°C Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.19
-
 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.2
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.29
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.3
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.64
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.99
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.2
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.2
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.3
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.5
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.7
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.7
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.7
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.9
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2.9
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
3
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
3.3
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
3.3
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
3.8
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
4.4
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
4.5
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
5.4
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
6.6
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
7.1
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
7.3
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
7.3
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
7.6
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
12
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
15
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
17
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
17
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
26
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
30
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
33
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
61
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
67
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
74
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
110
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
130
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
160
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
210
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
220
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
220
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
400
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
420
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
470
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Trametes ochracea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0037
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.005
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0058
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.008
-
 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0084
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.011
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0113
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.0166
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.024
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.046
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.053
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.059
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.062
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.094
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.27
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
0.54
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
4.7
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
7
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
8.2
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
15
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
37
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
43
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
63
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
130
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
140
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
180
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
180
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
200
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
230
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
280
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
290
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
330
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
340
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
360
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
370
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
420
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
480
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
500
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
510
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
520
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
520
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
940
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1200
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1210
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1250
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1640
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2100
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
2600
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
3000
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea