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show all sequences of 1.1.2.B5

Characterization of a novel PQQ-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea classified into auxiliary activities family 12 in carbohydrate-active enzymes

Takeda, K.; Matsumura, H.; Ishida, T.; Samejima, M.; Ohno, H.; Yoshida, M.; Igarashi, K.; Nakamura, N.; PLoS ONE 10, e0115722 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris
Coprinopsis cinerea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.9
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
23.1
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
24.8
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
30.3
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
49.7
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
66.8
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
84.7
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
236
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
363
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM Ca2+ is used in assay conditions
Coprinopsis cinerea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coprinopsis cinerea
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + 2,6-dichlorophenolindophenol
47.5% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucosone + ferricytochrome c
100% activity
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
D-lyxose + 2,6-dichlorophenolindophenol
13.4% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-talose + ferricytochrome c
24.8% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-fucose + 2,6-dichlorophenolindophenol
77.7% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-galactose + ferricytochrome c
65.4% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-glucose + ferricytochrome c
9.2% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-gulose + 2,6-dichlorophenolindophenol
52.1% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lyxose + 2,6-dichlorophenolindophenol
4.0% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-mannose + 2,6-dichlorophenolindophenol
3.6% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-rhamnose + 2,6-dichlorophenolindophenol
0.7% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-xylose + ferricytochrome c
11.9% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
additional information
the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation
741228
Coprinopsis cinerea
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12.9
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
17.8
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
22.4
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
24.9
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
35.5
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
53.3
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
56.1
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
56.4
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
74.1
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
Cofactor
Cofactor
Commentary
Organism
Structure
heme b
-
Coprinopsis cinerea
pyrroloquinoline quinone
-
Coprinopsis cinerea
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris
Coprinopsis cinerea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme b
-
Coprinopsis cinerea
pyrroloquinoline quinone
-
Coprinopsis cinerea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.9
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
23.1
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
24.8
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
30.3
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
49.7
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
66.8
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
84.7
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
236
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
363
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM Ca2+ is used in assay conditions
Coprinopsis cinerea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinose + 2,6-dichlorophenolindophenol
47.5% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucosone + ferricytochrome c
100% activity
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
D-lyxose + 2,6-dichlorophenolindophenol
13.4% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-talose + ferricytochrome c
24.8% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-fucose + 2,6-dichlorophenolindophenol
77.7% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-galactose + ferricytochrome c
65.4% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-glucose + ferricytochrome c
9.2% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
L-gulose + 2,6-dichlorophenolindophenol
52.1% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lyxose + 2,6-dichlorophenolindophenol
4.0% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-mannose + 2,6-dichlorophenolindophenol
3.6% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-rhamnose + 2,6-dichlorophenolindophenol
0.7% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-xylose + ferricytochrome c
11.9% activity compared to D-glucosone
741228
Coprinopsis cinerea
? + ferrocytochrome c
-
-
-
?
additional information
the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation
741228
Coprinopsis cinerea
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
12.9
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
17.8
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
22.4
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
24.9
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
35.5
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
53.3
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
56.1
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
56.4
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
74.1
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.09
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.19
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.63
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.77
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
1.13
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
1.17
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
2.27
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
9.37
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
L-Glucose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.09
-
L-Xylose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.19
-
D-Lyxose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.63
-
L-gulose
at pH 8.5 and 30°C
Coprinopsis cinerea
0.77
-
D-talose
at pH 8.5 and 30°C
Coprinopsis cinerea
1.13
-
L-galactose
at pH 8.5 and 30°C
Coprinopsis cinerea
1.17
-
D-arabinose
at pH 8.5 and 30°C
Coprinopsis cinerea
2.27
-
L-fucose
at pH 8.5 and 30°C
Coprinopsis cinerea
9.37
-
D-glucosone
at pH 8.5 and 30°C
Coprinopsis cinerea
Other publictions for EC 1.1.2.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739998
Takeda
pH-dependent electron transfer ...
Coprinopsis cinerea
Biochem. Biophys. Res. Commun.
477
369-373
2016
-
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-
-
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2
-
-
-
-
-
2
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2
-
-
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-
2
2
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2
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2
-
-
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2
-
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-
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-
-
-
-
-
-
2
-
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2
2
-
-
-
-
-
-
-
-
-
741228
Takeda
Characterization of a novel PQ ...
Coprinopsis cinerea
PLoS ONE
10
e0115722
2015
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1
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-
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-
9
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1
-
-
-
5
-
-
-
-
-
-
-
-
13
-
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-
9
-
-
-
2
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1
2
-
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9
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1
-
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13
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9
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9
9