BRENDA - Enzyme Database
show all sequences of 1.1.2.B3

Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U

Arias, S.; Olivera, E.R.; Arcos, M.; Naharro, G.; Luengo, J.M.; Environ. Microbiol. 10, 413-432 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene pedE, DNA and amino acid sequence determination and analysis
Pseudomonas putida
gene pedH, DNA and amino acid sequence determination and analysis
Pseudomonas putida
Engineering
Protein Variants
Commentary
Organism
additional information
analysis of Tn5 transposon insertion mutants, genetic organization, overview
Pseudomonas putida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
PedH
Pseudomonas putida
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64870
-
x * 64870, PedH, sequence calculation
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
phenylethanol + ferricytochrome c
Pseudomonas putida
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
phenylacetaldehyde + ferrocytochrome c
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
B1N7J0
PedE; gene pedE
-
Pseudomonas putida
B1N7J5
PedH; gene pedH
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
phenylethanol + ferricytochrome c
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
686425
Pseudomonas putida
phenylacetaldehyde + ferrocytochrome c
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 64870, PedH, sequence calculation
Pseudomonas putida
More
PedH contains a well-conserved PQQ-DH domain, structural analysis
Pseudomonas putida
Synonyms
Synonyms
Commentary
Organism
More
PedE does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
Pseudomonas putida
PedE
-
Pseudomonas putida
PedH
-
Pseudomonas putida
PQQ-alcohol dehydrogenase
-
Pseudomonas putida
PQQ-linked alcohol dehydrogenase
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no heme cofactor
Pseudomonas putida
pyrroloquinoline quinone
PQQ, dependent on, prosthetic group, the consensus sequences involved in the PQQ binding are GAGNPG in PedE, enzymes involved in PQQ biosynthesis are encoded by the pqq gene, e.g. pqqABCDEF, of the pqq cluster
Pseudomonas putida
pyrroloquinoline quinone
PQQ, dependent on, prosthetic group, the consensus sequences involved in the PQQ binding are GLGVQG and GSGVLG in PedH, enzymes involved in PQQ biosynthesis are encoded by the pqq gene, e.g. pqqABCDEF, of the pqq cluster
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pedE, DNA and amino acid sequence determination and analysis
Pseudomonas putida
gene pedH, DNA and amino acid sequence determination and analysis
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no heme cofactor
Pseudomonas putida
pyrroloquinoline quinone
PQQ, dependent on, prosthetic group, the consensus sequences involved in the PQQ binding are GAGNPG in PedE, enzymes involved in PQQ biosynthesis are encoded by the pqq gene, e.g. pqqABCDEF, of the pqq cluster
Pseudomonas putida
pyrroloquinoline quinone
PQQ, dependent on, prosthetic group, the consensus sequences involved in the PQQ binding are GLGVQG and GSGVLG in PedH, enzymes involved in PQQ biosynthesis are encoded by the pqq gene, e.g. pqqABCDEF, of the pqq cluster
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
analysis of Tn5 transposon insertion mutants, genetic organization, overview
Pseudomonas putida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
PedH
Pseudomonas putida
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64870
-
x * 64870, PedH, sequence calculation
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
phenylethanol + ferricytochrome c
Pseudomonas putida
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
phenylacetaldehyde + ferrocytochrome c
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
phenylethanol + ferricytochrome c
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
686425
Pseudomonas putida
phenylacetaldehyde + ferrocytochrome c
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 64870, PedH, sequence calculation
Pseudomonas putida
More
PedH contains a well-conserved PQQ-DH domain, structural analysis
Pseudomonas putida
Other publictions for EC 1.1.2.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
690646
Mennenga
Quinoprotein ethanol dehydroge ...
Pseudomonas aeruginosa
Arch. Microbiol.
191
361-367
2009
2
-
1
-
1
-
-
10
-
-
-
1
-
1
-
-
-
-
-
-
10
-
6
-
2
-
-
-
-
-
-
-
1
-
-
-
2
-
1
1
-
1
-
-
-
-
10
-
-
-
1
-
-
-
-
-
-
10
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686425
Arias
Genetic analyses and molecular ...
Pseudomonas putida
Environ. Microbiol.
10
413-432
2008
-
-
2
-
2
-
-
-
1
-
1
2
-
2
-
-
-
-
-
-
-
-
2
2
7
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
-
2
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688852
Schobert
A soluble two-component regula ...
Pseudomonas aeruginosa
Microbiology
147
363-372
2001
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-