Literature summary for 1.1.2.B2 extracted from

Harris, T.K.; Davidson, V.L.
A new kinetic model for the steady-state reactions of the quinoprotein methanol dehydrogenase from Paracoccus denitrificans (1993), Biochemistry, 32, 4362-4368.

Search for more literature for 1.1.2.B2

Activating Compound

Activating Compound	Comment	Organism	Structure
cyanide	suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview	Paracoccus denitrificans
NH4+	obligatory activator	Paracoccus denitrificans

Inhibitors

Inhibitors	Comment	Organism	Structure
cyanide	suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview	Paracoccus denitrificans
N,N,N',N'-tetramethyl-4-phenylenediamine	i.e. Wurster's blue	Paracoccus denitrificans
phenazine ethosulfate	the articficial cofactor exhibits strong substrate inhibition	Paracoccus denitrificans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.56	-	methanol	pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine	Paracoccus denitrificans
3.22	-	N,N,N',N'-tetramethyl-4-phenylenediamine	pH 9.0 30°C	Paracoccus denitrificans
3.39	-	ethanol	pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine	Paracoccus denitrificans
10.7	-	Propanol	pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine	Paracoccus denitrificans
11.8	-	phenazine ethosulfate	pH 9.0, 30°C	Paracoccus denitrificans
91.4	-	butanol	pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine	Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methanol + ferricytochrome c551i	Paracoccus denitrificans	-	formaldehyde + ferrocytochrome c551i	-	?

Organism

Organism	UniProt	Comment	Textmining
Paracoccus denitrificans	P12293	P12293 (large subunit, alpha) and P12293 (small subunit, beta)	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.6	-	-	Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butanol + N,N,N',N'-tetramethyl-4-phenylenediamine	-	Paracoccus denitrificans	butyraldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine	-	?
ethanol + N,N,N',N'-tetramethyl-4-phenylenediamine	-	Paracoccus denitrificans	acetaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine	-	?
methanol + 2,6-dichlorophenolindophenol	-	Paracoccus denitrificans	formaldehyde + reduced 2,6-dichlorophenolindophenol	-	?
methanol + ferricytochrome c551i	-	Paracoccus denitrificans	formaldehyde + ferrocytochrome c551i	-	?
methanol + N,N,N',N'-tetramethyl-4-phenylenediamine	-	Paracoccus denitrificans	formaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine	-	?
methanol + phenazine ethosulfate	phenazine ethosulfate is a two-electron acceptor	Paracoccus denitrificans	formaldehyde + reduced phenazine ethosulfate	-	?
propanol + N,N,N',N'-tetramethyl-4-phenylenediamine	-	Paracoccus denitrificans	propionaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine	-	?

Synonyms

Synonyms	Comment	Organism
quinoprotein methanol dehydrogenase	-	Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Paracoccus denitrificans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Paracoccus denitrificans

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	-	Paracoccus denitrificans

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.59	-	phenazine ethosulfate	pH 9.0, 30°C	Paracoccus denitrificans
9.37	-	N,N,N',N'-tetramethyl-4-phenylenediamine	pH 9.0 30°C	Paracoccus denitrificans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)