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Literature summary for 1.1.2.8 extracted from
- Quinoprotein ethanol dehydrogenase of Pseudomonas aeruginosa is a homodimer. Sequence of the gene and deduced structural properties of the enzyme (1998), Eur. J. Biochem., 257, 409-419.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| alignment with the amino acid sequence of the large subunit of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens. The amino acid residues involved in the binding of pyrroloquinoline quinone and Ca2+ at the active site are conserved | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | In the presence of the prosthetic group, expression of the Pseudomonas gene encoding the 60-kDa subunit of quinoprotein ethanol dehydrogenase in Escherichia coli results in formation of active enzyme | Pseudomonas aeruginosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9000 | - |
1 * 60000 + 1 * 9000, SDS-PAGE | Pseudomonas aeruginosa |
| 60000 | - |
1 * 60000 + 1 * 9000, SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9Z4J7 | large subunit | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | sequence contains a signal peptide of 34 residues | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | ethanal + reduced 2,6-dichlorophenolindophenol | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 60000 + 1 * 9000, SDS-PAGE | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Pseudomonas aeruginosa | |
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