Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Ca2+-lacking, catalytically inactive mutant apoenzymes, reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer | Methylorubrum extorquens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Methylorubrum extorquens | - |
- |
soluble | - |
Methylorubrum extorquens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Ba2+ shows 102% of the activity with Ca2+ | Methylorubrum extorquens | |
Ca2+ | 2 Ca2+ ions are irreversibly incorporated per alpha2beta2 tetramer. Calcium can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+ | Methylorubrum extorquens | |
Sr2+ | Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Sr2+ shows 94% of the activity with Ca2+ | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + ferricytochrome cL | Methylorubrum extorquens | - |
formaldehyde + ferrocytochrome cL | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta); gene mxaA | - |
Renatured (Comment) | Organism |
---|---|
reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer, time course of Ca2+ incorporation, overview | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + ferricytochrome cL | - |
Methylorubrum extorquens | formaldehyde + ferrocytochrome cL | - |
? |
Synonyms | Comment | Organism |
---|---|---|
quinoprotein methanol dehydrogenase | - |
Methylorubrum extorquens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
about | Methylorubrum extorquens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.5 | pH profile, overview | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome cL | electron acceptor | Methylorubrum extorquens | |
pyrroloquinoline quinone | PQQ, prosthetic group | Methylorubrum extorquens |