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Literature summary for 1.1.2.7 extracted from

  • Avezoux, A.; Goodwin, M.G.; Anthony, C.
    The role of the novel disulphide ring in the active site of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens (1995), Biochem. J., 307, 735-741.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information a Ca2+-free enzyme mxaA mutant is inactive Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ one molecule per enzyme alpha-subunit. Neither the quinone form of pyrroloquinoline quinone, nor the disulfide ring or its reduced form are absolutely essential for calcium incorporation into the active site Methylorubrum extorquens
Fe2+ a cytochrome protein Methylorubrum extorquens

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens P16027 and P14775 P16027 (large subunit, alpha) and P14775 (small subunit, beta)
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Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 P16027 and P14775 P16027 (large subunit, alpha) and P14775 (small subunit, beta)
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + ferricytochrome cL the reaction includes an electron transfer from the quinol PQQH2 to the electron acceptor cytochrome CL mediated by the disulfide structure of the active site, the active site of MDH involves Trp243 that forms the base of the active site chamber, and the Cys103-Cys104 disulfide ring and the pyrroloquinoline quinone prosthetic group, which is in the semiquinone form having the oxygen of the C4 carbonyl displaced out of the plane of the ring, overview Methylorubrum extorquens formaldehyde + ferrocytochrome cL
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?
methanol + ferricytochrome cL the reaction includes an electron transfer from the quinol PQQH2 to the electron acceptor cytochrome CL mediated by the disulfide structure of the active site, the active site of MDH involves Trp243 that forms the base of the active site chamber, and the Cys103-Cys104 disulfide ring and the pyrroloquinoline quinone prosthetic group, which is in the semiquinone form having the oxygen of the C4 carbonyl displaced out of the plane of the ring, overview Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 formaldehyde + ferrocytochrome cL
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?

Synonyms

Synonyms Comment Organism
methanol dehydrogenase
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Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
cytochrome cL
-
Methylorubrum extorquens
additional information an NAD(P)-independent enzyme Methylorubrum extorquens
pyrroloquinoline quinone PQQ Methylorubrum extorquens