BRENDA - Enzyme Database
show all sequences of 1.1.2.4

Laboratory prototype of bioreactor for oxidation of toxic D-lactate using yeast cells overproducing D-lactate cytochrome c oxidoreductase

Karkovska, M.; Smutok, O.; Gonchar, M.; BioMed Res. Int. 2016, 4652876 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene DLD1, 6fold overexpression of DLDH under the strong constitutive promoter prAOX in Hansenula polymorpha strain DELTAcyb2 tr6, a strain with impairment in glucose repression of AOX promoter and devoid of catalase and L-lactate-cytochrome c oxidoreductase activities
Ogataea angusta
Engineering
Protein Variants
Commentary
Organism
additional information
overexpression of DLDH coupling with the deletion of L-lactate-cytochrome c oxidoreductase activity opens possibility for usage of the strain as a base for construction of bioreactor for removing toxic D-lactate from fermented products due to oxidation to nontoxic pyruvate. Construction and evaluation of a bioreactor for oxidation of toxic D-lactate using yeast cells overproducing D-lactate cytochrome c oxidoreductase, using permeabilized cells and alginate gel. The efficiency of enzymatic oxidation of D-lactate by the bioreactor prototype tightly depends on flow rate through the column
Ogataea angusta
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Ogataea angusta
5739
-
Organism
Organism
UniProt
Commentary
Textmining
Ogataea angusta
W1QLN6
-
-
Ogataea angusta tr6
W1QLN6
-
-
Synonyms
Synonyms
Commentary
Organism
D-lactate cytochrome c oxidoreductase
-
Ogataea angusta
D-lactate dehydrogenase (cytochrome)
-
Ogataea angusta
D-lactate: cytochrome c oxidoreductase
-
Ogataea angusta
DLD1
-
Ogataea angusta
DLDH
-
Ogataea angusta
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at, in permeabilized cells
Ogataea angusta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Ogataea angusta
Cloned(Commentary) (protein specific)
Commentary
Organism
gene DLD1, 6fold overexpression of DLDH under the strong constitutive promoter prAOX in Hansenula polymorpha strain DELTAcyb2 tr6, a strain with impairment in glucose repression of AOX promoter and devoid of catalase and L-lactate-cytochrome c oxidoreductase activities
Ogataea angusta
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
overexpression of DLDH coupling with the deletion of L-lactate-cytochrome c oxidoreductase activity opens possibility for usage of the strain as a base for construction of bioreactor for removing toxic D-lactate from fermented products due to oxidation to nontoxic pyruvate. Construction and evaluation of a bioreactor for oxidation of toxic D-lactate using yeast cells overproducing D-lactate cytochrome c oxidoreductase, using permeabilized cells and alginate gel. The efficiency of enzymatic oxidation of D-lactate by the bioreactor prototype tightly depends on flow rate through the column
Ogataea angusta
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Ogataea angusta
5739
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at, in permeabilized cells
Ogataea angusta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Ogataea angusta
Other publictions for EC 1.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
BioMed Res. Int.
2016
4652876
2016
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743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
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1
1
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743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
2014
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1
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740447
de Bari
Prostate cancer cells metaboli ...
Homo sapiens
FEBS Lett.
587
467-473
2013
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2
2
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698932
Engqvist
Two D-2-hydroxy-acid dehydroge ...
Arabidopsis thaliana
J. Biol. Chem.
284
25026-25037
2009
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1
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1
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1
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8
1
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5
1
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1
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1
1
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5
5
706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
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1
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685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
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703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
-
1
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672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
1
1
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1
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1
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1
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684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
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1
1
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3
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
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1
2
1
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1
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2
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1
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1
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
-
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1
1
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2
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1
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2
1
1
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1
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1
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1
1
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389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
-
1
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1
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2
1
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2
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389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
-
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1
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1
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389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
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1
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2
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
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5
1
1
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389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
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1
1
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2
1
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1
1
2
1
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389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
-
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2
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1
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1
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1
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287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
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4
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389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
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1
3
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3
1
1
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1
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389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
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1
4
4
1
1
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3
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3
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1
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1
1
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1
3
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1
2
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3
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389625
Nygaard
-
Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
7
557-565
1963
-
-
-
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1
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3
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1
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1
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389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
-
-
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3
3
1
1
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2
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1
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5
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1
2
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2
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2
3
1
1
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1
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5
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1
2
2
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