BRENDA - Enzyme Database
show all sequences of 1.1.2.4

Prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells

de Bari, L.; Moro, L.; Passarella, S.; FEBS Lett. 587, 467-473 (2013)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
oxalate
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
the enzyme is located on the inner face of the inner mitochondrial membrane
Homo sapiens
5743
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Homo sapiens
-
pyruvate + 2 ferrocytochrome c
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
Q86WU2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
PC-3 cell
-
Homo sapiens
-
PNT-1A cell
-
Homo sapiens
-
prostate cancer cell
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-lactate + 2 ferricytochrome c
-
740447
Homo sapiens
pyruvate + 2 ferrocytochrome c
-
-
-
?
additional information
D-LDH activity assay and (R)-lactate-dependent cyt c and flavin reduction
740447
Homo sapiens
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
D-LDH
-
Homo sapiens
DLD
-
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
the enzyme is a flavoprotein. The flavin/pyridine cofactor redox state is detected fluorimetrically in cell homogenates
Homo sapiens
ferricytochrome c
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
the enzyme is a flavoprotein. The flavin/pyridine cofactor redox state is detected fluorimetrically in cell homogenates
Homo sapiens
ferricytochrome c
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
oxalate
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrial inner membrane
the enzyme is located on the inner face of the inner mitochondrial membrane
Homo sapiens
5743
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Homo sapiens
-
pyruvate + 2 ferrocytochrome c
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
PC-3 cell
-
Homo sapiens
-
PNT-1A cell
-
Homo sapiens
-
prostate cancer cell
-
Homo sapiens
-
prostate gland
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
-
740447
Homo sapiens
pyruvate + 2 ferrocytochrome c
-
-
-
?
additional information
D-LDH activity assay and (R)-lactate-dependent cyt c and flavin reduction
740447
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
metabolism
prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria being twofold higher in cancer compared to healthy cells, overview
Homo sapiens
physiological function
the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria being twofold higher in cancer compared to healthy cells, overview
Homo sapiens
physiological function
the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner
Homo sapiens
Other publictions for EC 1.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
BioMed Res. Int.
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4652876
2016
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743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
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743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
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740447
de Bari
Prostate cancer cells metaboli ...
Homo sapiens
FEBS Lett.
587
467-473
2013
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1
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698932
Engqvist
Two D-2-hydroxy-acid dehydroge ...
Arabidopsis thaliana
J. Biol. Chem.
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25026-25037
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1
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1
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1
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1
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5
5
706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
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685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
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703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
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1
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672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
1
1
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1
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684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
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3
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
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1
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389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
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1
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389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
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1
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389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
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389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
-
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389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
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2
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287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
-
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1
4
1
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1
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1
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3
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389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
-
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1
3
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3
1
1
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1
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389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
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1
4
4
1
1
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3
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3
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3
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1
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4
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4
1
1
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1
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1
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1
1
3
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1
2
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3
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389625
Nygaard
-
Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
7
557-565
1963
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1
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3
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1
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1
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4
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4
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4
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1
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3
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1
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4
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389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
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3
3
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1
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1
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2
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1
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1
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5
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1
2
2
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3
2
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3
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3
2
3
1
1
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1
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1
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1
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5
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1
2
2
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