BRENDA - Enzyme Database
show all sequences of 1.1.2.4

Amperometric biosensor for D-lactate assay

Pohanka, M.; Zboril, P.; Food Technol. Biotechnol. 46, 107-110 (2008)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
carbon paste electrode-based biosensor with immobilized D-LCR seems to be optimal for analysis, representing significant advantage due to simplification of the whole device, i.e., the sensor exerts lower limit of detection under supposed concentration of D-lactate in real samples, low material demands, simplicity of D-LCR biosensor and short total time of analysis of about 2 min, pointing at the sensor possibilities in commercial applications
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by affinity chromatography
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-20C
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-lactate + 2 ferricytochrome c
substrate specific for D-LCR. D-LCR is efficient enough in transferring electrons from D-lactate to the next mediators
703801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
D-lactate cytochrome c oxidoreductase
-
Saccharomyces cerevisiae
D-LCR
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
Application (protein specific)
Application
Commentary
Organism
additional information
carbon paste electrode-based biosensor with immobilized D-LCR seems to be optimal for analysis, representing significant advantage due to simplification of the whole device, i.e., the sensor exerts lower limit of detection under supposed concentration of D-lactate in real samples, low material demands, simplicity of D-LCR biosensor and short total time of analysis of about 2 min, pointing at the sensor possibilities in commercial applications
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
by affinity chromatography
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-20C
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-lactate + 2 ferricytochrome c
substrate specific for D-LCR. D-LCR is efficient enough in transferring electrons from D-lactate to the next mediators
703801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
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743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
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743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
2014
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Prostate cancer cells metaboli ...
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Two D-2-hydroxy-acid dehydroge ...
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706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
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685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
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703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
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672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
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105-110
2007
1
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684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
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389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
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389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
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389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
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389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
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389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
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287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
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389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
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389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
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389625
Nygaard
-
Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
7
557-565
1963
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389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
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