BRENDA - Enzyme Database
show all sequences of 1.1.2.4

Two D-2-hydroxy-acid dehydrogenases in Arabidopsis thaliana with catalytic capacities to participate in the last reactions of the methylglyoxal and beta-oxidation pathways

Engqvist, M.; Drincovich, M.F.; Fluegge, U.I.; Maurino, V.G.; J. Biol. Chem. 284, 25026-25037 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
additional information
mRNAs are present in both d2hgdh mutant lines, but not in dldh1-1 and dldh1-2 mutant lines
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
0.164
-
D-lactate
-
Arabidopsis thaliana
0.432
-
glycolate
-
Arabidopsis thaliana
4.486
-
L-lactate
-
Arabidopsis thaliana
8.871
-
D-glycerate
-
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
2 * 59000, sequence analysis
Arabidopsis thaliana
135000
-
gel filtration
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q94AX4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-2-hydroxybutyrate + ferricytochrome c
135% relative activity compared to (R)lactate, highest catalytic efficiency, prefered substrate
698932
Arabidopsis thaliana
?
-
-
-
?
D-2-hydroxyglutarate + ferricytochrome c
0.15% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
D-glycerate + ferricytochrome c
9.2% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
D-lactate + 2 ferricytochrome c
100% relative activity, prefered substrate, with a high catalytic efficiency 200- and 2000fold higher than that for L-lactate and glycolate, respectively
698932
Arabidopsis thaliana
pyruvate + 2 ferrocytochrome c
-
-
-
?
DL-glycerate + ferricytochrome c
10.3% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
glycolate + ferricytochrome c
0.15% relative activity compared to (R)lactate, O2 does not function as the acceptor for glycolate electrons
698932
Arabidopsis thaliana
?
-
-
-
?
L-lactate + ferricytochrome c
10.8% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
additional information
no activity with propionate, acetate, glutarate, DL-3-hydroxybutyrate and 4-hydroxybutyrate
698932
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 59000, sequence analysis
Arabidopsis thaliana
Synonyms
Synonyms
Commentary
Organism
D-lactate cytochrome c oxidoreductase
-
Arabidopsis thaliana
D-LDH
-
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00167
-
glycolate
-
Arabidopsis thaliana
0.1
-
D-glycerate
-
Arabidopsis thaliana
0.1167
-
L-lactate
-
Arabidopsis thaliana
1.083
-
D-lactate
-
Arabidopsis thaliana
1.467
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
pH optimum for D-lactate, L-lactate and glycolate oxidation
Arabidopsis thaliana
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.25
-
retains at least 25% activity down to pH 7.25, whereas it rapidly loses activity at lower pH values
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Arabidopsis thaliana
additional information
no activity with NAD+ or NADP+ as electron acceptors
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Arabidopsis thaliana
additional information
no activity with NAD+ or NADP+ as electron acceptors
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
mRNAs are present in both d2hgdh mutant lines, but not in dldh1-1 and dldh1-2 mutant lines
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
0.164
-
D-lactate
-
Arabidopsis thaliana
0.432
-
glycolate
-
Arabidopsis thaliana
4.486
-
L-lactate
-
Arabidopsis thaliana
8.871
-
D-glycerate
-
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
2 * 59000, sequence analysis
Arabidopsis thaliana
135000
-
gel filtration
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-2-hydroxybutyrate + ferricytochrome c
135% relative activity compared to (R)lactate, highest catalytic efficiency, prefered substrate
698932
Arabidopsis thaliana
?
-
-
-
?
D-2-hydroxyglutarate + ferricytochrome c
0.15% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
D-glycerate + ferricytochrome c
9.2% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
D-lactate + 2 ferricytochrome c
100% relative activity, prefered substrate, with a high catalytic efficiency 200- and 2000fold higher than that for L-lactate and glycolate, respectively
698932
Arabidopsis thaliana
pyruvate + 2 ferrocytochrome c
-
-
-
?
DL-glycerate + ferricytochrome c
10.3% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
glycolate + ferricytochrome c
0.15% relative activity compared to (R)lactate, O2 does not function as the acceptor for glycolate electrons
698932
Arabidopsis thaliana
?
-
-
-
?
L-lactate + ferricytochrome c
10.8% relative activity compared to (R)lactate
698932
Arabidopsis thaliana
?
-
-
-
?
additional information
no activity with propionate, acetate, glutarate, DL-3-hydroxybutyrate and 4-hydroxybutyrate
698932
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 59000, sequence analysis
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00167
-
glycolate
-
Arabidopsis thaliana
0.1
-
D-glycerate
-
Arabidopsis thaliana
0.1167
-
L-lactate
-
Arabidopsis thaliana
1.083
-
D-lactate
-
Arabidopsis thaliana
1.467
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
pH optimum for D-lactate, L-lactate and glycolate oxidation
Arabidopsis thaliana
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.25
-
retains at least 25% activity down to pH 7.25, whereas it rapidly loses activity at lower pH values
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
physiological function
participates in the methylglyoxal pathway
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
participates in the methylglyoxal pathway
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0033
-
glycolate
-
Arabidopsis thaliana
0.0167
-
D-glycerate
-
Arabidopsis thaliana
0.033
-
L-lactate
-
Arabidopsis thaliana
6.583
-
D-lactate
-
Arabidopsis thaliana
24.17
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0033
-
glycolate
-
Arabidopsis thaliana
0.0167
-
D-glycerate
-
Arabidopsis thaliana
0.033
-
L-lactate
-
Arabidopsis thaliana
6.583
-
D-lactate
-
Arabidopsis thaliana
24.17
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
Other publictions for EC 1.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
BioMed Res. Int.
2016
4652876
2016
-
-
1
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
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5
1
-
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1
-
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1
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1
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1
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1
-
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-
1
-
-
-
-
-
-
-
-
-
743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
-
-
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
3
-
-
3
1
5
-
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2
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1
2
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1
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1
1
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3
-
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3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
2014
-
-
1
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1
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2
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7
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2
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1
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740447
de Bari
Prostate cancer cells metaboli ...
Homo sapiens
FEBS Lett.
587
467-473
2013
-
-
-
-
-
-
1
-
1
-
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1
-
1
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4
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2
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2
1
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2
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1
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1
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1
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4
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
698932
Engqvist
Two D-2-hydroxy-acid dehydroge ...
Arabidopsis thaliana
J. Biol. Chem.
284
25026-25037
2009
-
-
1
-
1
-
-
5
-
-
2
-
-
4
-
-
1
-
-
-
-
-
8
1
2
-
-
-
5
1
-
1
2
-
-
-
-
-
1
2
-
1
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
8
1
-
-
-
5
1
-
1
-
-
1
1
-
5
5
706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
-
-
-
-
-
-
-
-
1
-
-
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1
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1
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4
-
3
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1
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1
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4
-
3
-
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-
-
-
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685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
-
-
-
-
-
-
-
1
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1
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1
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3
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3
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1
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3
-
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-
-
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-
-
-
-
-
-
-
703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
-
1
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
1
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2
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1
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1
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1
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1
1
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1
-
-
-
-
-
-
-
-
-
672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
1
1
-
-
-
-
-
-
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1
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1
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1
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2
1
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1
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1
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1
1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
-
-
-
-
-
-
-
1
1
-
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1
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1
1
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1
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1
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3
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1
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1
1
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1
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3
-
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
-
-
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1
2
1
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1
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1
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1
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1
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1
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2
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1
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1
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
-
-
-
-
-
1
1
-
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2
-
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1
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1
-
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2
1
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1
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1
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1
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1
1
1
1
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1
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1
1
1
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2
-
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1
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2
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
-
1
-
1
-
-
-
2
1
-
1
-
2
-
-
-
-
-
-
-
-
2
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1
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1
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1
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1
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1
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2
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389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
-
-
1
-
1
-
-
-
1
-
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1
-
3
-
-
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-
-
-
-
-
2
-
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1
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1
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1
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1
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2
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389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
-
-
1
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2
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1
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4
-
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2
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2
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
-
-
-
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5
1
1
-
-
1
-
1
-
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1
-
-
-
-
1
3
-
-
-
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1
1
-
4
-
-
-
1
-
-
4
-
-
-
-
5
-
1
1
-
-
1
-
-
-
1
-
-
-
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
-
-
-
-
-
1
1
1
-
3
2
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
3
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
-
-
-
-
-
-
2
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
-
-
-
-
-
-
1
4
1
4
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
4
1
4
1
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
-
-
-
-
-
-
1
3
-
3
1
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
3
-
3
1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
-
-
-
-
-
1
4
4
1
1
-
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
-
-
1
2
-
3
3
-
-
-
-
-
-
3
-
-
1
-
4
-
4
1
1
-
1
-
-
-
1
-
-
1
1
3
-
-
-
-
1
2
-
3
-
-
-
-
-
-
-
389625
Nygaard
-
Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
7
557-565
1963
-
-
-
-
-
-
1
-
1
3
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
4
-
-
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-
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-
4
-
-
-
-
1
-
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1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
-
-
-
-
-
-
3
3
1
1
-
1
-
2
-
-
1
-
-
-
1
-
5
-
-
-
-
-
1
2
2
-
3
2
-
-
-
-
-
3
-
-
-
-
3
2
3
1
1
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
1
2
2
-
-
-
-
-
-
-
-