BRENDA - Enzyme Database
show all sequences of 1.1.2.4

D-Lactate dehydrogenase of Desulfovibrio vulgaris

Ogata, M.; Arihara, K.; Yagi, T.; J. Biochem. 89, 1423-1431 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
slightly stimulating
Desulfovibrio vulgaris
Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Desulfovibrio vulgaris
EDTA
no inhibitor
Desulfovibrio vulgaris
o-phenanthroline
very strong
Desulfovibrio vulgaris
oxalate
-
Desulfovibrio vulgaris
p-chloromercuribenzoate
weak
Desulfovibrio vulgaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
D-lactate
-
Desulfovibrio vulgaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
equally distributed between soluble and membrane fraction
Desulfovibrio vulgaris
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Desulfovibrio vulgaris
required for growth on lactate as sole carbon source
pyruvate + ferrocytochrome c
-
Desulfovibrio vulgaris
?
Organism
Organism
UniProt
Commentary
Textmining
Desulfovibrio vulgaris
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Desulfovibrio vulgaris
Storage Stability
Storage Stability
Organism
4°C, 0.1 M Tris-buffer, pH 7.3, 20% loss of activity
Desulfovibrio vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-lactate + 2 ferricytochrome c
required for growth on lactate as sole carbon source
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c
-
389630
Desulfovibrio vulgaris
?
(R)-lactate + ferricytochrome c
traces of activity with L-isomer
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c
-
389630
Desulfovibrio vulgaris
?
D-2-hydroxybutyrate + cytochrome c
-
389630
Desulfovibrio vulgaris
2-oxobutanoate + ferrocytochrome c
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
-
Desulfovibrio vulgaris
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
no activity at pH 5, almost maximum activity at pH 9
Desulfovibrio vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Desulfovibrio vulgaris
cytochrome c
only cytochrome c-553 from same organism, eucaryotic cytochromes are not reduced
Desulfovibrio vulgaris
additional information
no activity with NAD+ or NADP+
Desulfovibrio vulgaris
phenazine methosulfate
can substitute for cytochrome c
Desulfovibrio vulgaris
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
slightly stimulating
Desulfovibrio vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Desulfovibrio vulgaris
cytochrome c
only cytochrome c-553 from same organism, eucaryotic cytochromes are not reduced
Desulfovibrio vulgaris
additional information
no activity with NAD+ or NADP+
Desulfovibrio vulgaris
phenazine methosulfate
can substitute for cytochrome c
Desulfovibrio vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Desulfovibrio vulgaris
EDTA
no inhibitor
Desulfovibrio vulgaris
o-phenanthroline
very strong
Desulfovibrio vulgaris
oxalate
-
Desulfovibrio vulgaris
p-chloromercuribenzoate
weak
Desulfovibrio vulgaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
D-lactate
-
Desulfovibrio vulgaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
equally distributed between soluble and membrane fraction
Desulfovibrio vulgaris
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Desulfovibrio vulgaris
required for growth on lactate as sole carbon source
pyruvate + ferrocytochrome c
-
Desulfovibrio vulgaris
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.1 M Tris-buffer, pH 7.3, 20% loss of activity
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
required for growth on lactate as sole carbon source
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c
-
389630
Desulfovibrio vulgaris
?
(R)-lactate + ferricytochrome c
traces of activity with L-isomer
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c
-
389630
Desulfovibrio vulgaris
?
D-2-hydroxybutyrate + cytochrome c
-
389630
Desulfovibrio vulgaris
2-oxobutanoate + ferrocytochrome c
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
-
Desulfovibrio vulgaris
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
no activity at pH 5, almost maximum activity at pH 9
Desulfovibrio vulgaris
Other publictions for EC 1.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
BioMed Res. Int.
2016
4652876
2016
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1
-
1
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-
1
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7
-
-
-
-
-
-
-
-
-
-
5
1
-
-
-
1
-
-
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1
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1
-
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-
-
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1
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-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
-
-
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
3
-
-
3
1
5
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
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1
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1
1
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3
-
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3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
2014
-
-
1
-
1
-
-
-
-
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2
-
7
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2
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3
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1
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1
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1
1
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1
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2
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2
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-
1
-
-
-
-
-
-
-
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-
740447
de Bari
Prostate cancer cells metaboli ...
Homo sapiens
FEBS Lett.
587
467-473
2013
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
2
1
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1
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2
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2
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1
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1
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1
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4
-
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2
-
1
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1
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-
-
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2
2
-
-
-
698932
Engqvist
Two D-2-hydroxy-acid dehydroge ...
Arabidopsis thaliana
J. Biol. Chem.
284
25026-25037
2009
-
-
1
-
1
-
-
5
-
-
2
-
-
4
-
-
1
-
-
-
-
-
8
1
2
-
-
-
5
1
-
1
2
-
-
-
-
-
1
2
-
1
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
8
1
-
-
-
5
1
-
1
-
-
1
1
-
5
5
706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
-
-
-
-
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-
-
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1
-
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1
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1
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4
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3
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4
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3
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-
-
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-
-
685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
-
-
-
-
-
-
-
1
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1
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1
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3
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1
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3
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-
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-
-
-
-
703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
-
1
-
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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1
1
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1
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-
-
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-
672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
1
1
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
-
-
-
-
-
-
-
1
1
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1
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1
1
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1
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3
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1
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1
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1
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3
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
-
-
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1
2
1
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1
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1
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1
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1
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1
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2
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1
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1
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
-
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1
1
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2
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1
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1
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1
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1
1
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2
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1
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2
1
1
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1
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1
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1
1
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-
389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
-
1
-
1
-
-
-
2
1
-
1
-
2
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2
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1
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2
1
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1
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2
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-
-
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-
-
-
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389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
-
-
1
-
1
-
-
-
1
-
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1
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3
-
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2
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389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
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1
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2
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1
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4
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
-
-
-
-
-
5
1
1
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1
-
1
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1
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1
3
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1
1
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4
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1
1
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1
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1
3
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1
1
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389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
-
-
-
-
-
1
1
1
-
3
2
1
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1
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1
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1
1
2
1
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1
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1
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1
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1
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1
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3
2
1
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1
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1
1
2
1
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389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
-
-
-
-
-
-
2
-
-
1
-
1
-
1
-
-
-
-
-
-
-
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3
-
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-
-
-
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2
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2
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2
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1
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1
-
-
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3
-
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-
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-
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-
-
-
-
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-
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287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
-
-
-
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1
4
1
4
1
1
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1
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3
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4
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4
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1
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4
1
4
1
1
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3
-
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389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
-
-
-
-
-
-
1
3
-
3
1
1
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1
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1
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-
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3
-
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1
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3
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3
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1
-
3
-
3
1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
-
-
-
-
-
1
4
4
1
1
-
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
-
-
1
2
-
3
3
-
-
-
-
-
-
3
-
-
1
-
4
-
4
1
1
-
1
-
-
-
1
-
-
1
1
3
-
-
-
-
1
2
-
3
-
-
-
-
-
-
-
389625
Nygaard
-
Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
7
557-565
1963
-
-
-
-
-
-
1
-
1
3
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
-
1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
-
-
-
-
-
-
3
3
1
1
-
1
-
2
-
-
1
-
-
-
1
-
5
-
-
-
-
-
1
2
2
-
3
2
-
-
-
-
-
3
-
-
-
-
3
2
3
1
1
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
1
2
2
-
-
-
-
-
-
-
-