BRENDA - Enzyme Database
show all sequences of 1.1.2.4

D(-)-lactic cytochrome c reductase, a flavoprotein from yeast

Nygaard, A.P.; J. Biol. Chem. 236, 920-925 (1961)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
DL-glycerate
strong inhibition
Saccharomyces cerevisiae
oxalate
competitive
Saccharomyces cerevisiae
oxaloacetate
competitive
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
D-lactate
-
Saccharomyces cerevisiae
1.34
-
D-2-hydroxybutyrate
-
Saccharomyces cerevisiae
2.5
-
D-2-hydroxyvalerate
-
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
ferricyanide
can substitute for cytochrome c
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c
-
Saccharomyces cerevisiae
-
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
87
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-lactate + 2 ferricytochrome c
-
389624
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
389624
Saccharomyces cerevisiae
-
(R)-lactate + ferricytochrome c
-
389624
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
389624
Saccharomyces cerevisiae
-
D-2-hydroxybutyrate + cytochrome c
-
389624
Saccharomyces cerevisiae
2-oxobutanoate + ferrocytochrome c
-
-
-
?
D-2-hydroxyvalerate + cytochrome c
very poor substrate
389624
Saccharomyces cerevisiae
2-oxovalerate + ferrocytochrome c
-
-
-
?
DL-glycerate + ferricyanide
10% of the rate of D-lactate oxidation
389624
Saccharomyces cerevisiae
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
250
-
D-lactate
per mol flavin
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
with ferricyanide
Saccharomyces cerevisiae
7.5
-
with cytochrome c
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
very low activity with ferricyanide
Saccharomyces cerevisiae
5.5
8.5
low activity with cytochrome c at pH 5.5, almost maximum activity at pH 8.5
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Saccharomyces cerevisiae
cytochrome c
-
Saccharomyces cerevisiae
FAD
-
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0016
-
oxalate
-
Saccharomyces cerevisiae
0.01
-
oxaloacetate
-
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Saccharomyces cerevisiae
cytochrome c
-
Saccharomyces cerevisiae
FAD
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DL-glycerate
strong inhibition
Saccharomyces cerevisiae
oxalate
competitive
Saccharomyces cerevisiae
oxaloacetate
competitive
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0016
-
oxalate
-
Saccharomyces cerevisiae
0.01
-
oxaloacetate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
D-lactate
-
Saccharomyces cerevisiae
1.34
-
D-2-hydroxybutyrate
-
Saccharomyces cerevisiae
2.5
-
D-2-hydroxyvalerate
-
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
ferricyanide
can substitute for cytochrome c
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c
-
Saccharomyces cerevisiae
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
87
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-lactate + 2 ferricytochrome c
-
389624
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
389624
Saccharomyces cerevisiae
-
(R)-lactate + ferricytochrome c
-
389624
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
389624
Saccharomyces cerevisiae
-
D-2-hydroxybutyrate + cytochrome c
-
389624
Saccharomyces cerevisiae
2-oxobutanoate + ferrocytochrome c
-
-
-
?
D-2-hydroxyvalerate + cytochrome c
very poor substrate
389624
Saccharomyces cerevisiae
2-oxovalerate + ferrocytochrome c
-
-
-
?
DL-glycerate + ferricyanide
10% of the rate of D-lactate oxidation
389624
Saccharomyces cerevisiae
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
250
-
D-lactate
per mol flavin
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
with ferricyanide
Saccharomyces cerevisiae
7.5
-
with cytochrome c
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
very low activity with ferricyanide
Saccharomyces cerevisiae
5.5
8.5
low activity with cytochrome c at pH 5.5, almost maximum activity at pH 8.5
Saccharomyces cerevisiae
Other publictions for EC 1.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742052
Karkovska
Laboratory prototype of biore ...
Ogataea angusta, Ogataea angusta tr6
BioMed Res. Int.
2016
4652876
2016
-
-
1
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
5
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
743500
Welchen
D-Lactate dehydrogenase links ...
Arabidopsis thaliana
Plant Physiol.
172
901-912
2016
-
-
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
3
-
-
3
1
5
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
3
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743861
Smutok
D-Lactate-selective amperomet ...
Ogataea angusta, Ogataea angusta tr6
Talanta
125
227-232
2014
-
-
1
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740447
de Bari
Prostate cancer cells metaboli ...
Homo sapiens
FEBS Lett.
587
467-473
2013
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
4
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
698932
Engqvist
Two D-2-hydroxy-acid dehydroge ...
Arabidopsis thaliana
J. Biol. Chem.
284
25026-25037
2009
-
-
1
-
1
-
-
5
-
-
2
-
-
4
-
-
1
-
-
-
-
-
8
1
2
-
-
-
5
1
-
1
2
-
-
-
-
-
1
2
-
1
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
8
1
-
-
-
5
1
-
1
-
-
1
1
-
5
5
706465
Pohanka
-
D-Lactate dehydrogenase (cytoc ...
Saccharomyces cerevisiae
Pol. J. Chem.
83
415-420
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
4
-
3
-
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-
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1
-
-
-
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1
-
-
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685358
Mourier
Kinetic activation of yeast mi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1777
1283-1288
2008
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
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3
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1
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1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703801
Pohanka
-
Amperometric biosensor for D-l ...
Saccharomyces cerevisiae
Food Technol. Biotechnol.
46
107-110
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
-
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1
1
-
2
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1
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1
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1
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1
1
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1
-
-
-
-
-
-
-
-
-
672749
Hao
Pseudomonas stutzeri as a nove ...
Pseudomonas stutzeri
Biotechnol. Lett.
29
105-110
2007
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
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1
-
2
1
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1
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1
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1
1
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1
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1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684629
Ma
Membrane-bound L- and D-lactat ...
Pseudomonas stutzeri
Appl. Microbiol. Biotechnol.
77
91-98
2007
-
-
-
-
-
-
-
1
1
-
-
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1
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1
1
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1
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1
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3
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1
1
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1
1
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1
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3
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672323
Atlante
Transport and metabolism of D- ...
Helianthus tuberosus
Biochim. Biophys. Acta
1708
13-22
2005
-
-
-
-
-
-
1
2
1
-
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1
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1
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2
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1
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1
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2
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2
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1
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2
1
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1
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2
-
1
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1
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655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
-
-
-
-
-
1
1
-
-
2
-
-
1
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-
1
-
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2
1
-
1
-
1
-
1
-
1
1
1
1
-
-
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-
1
-
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-
1
1
1
-
-
2
-
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
389633
Lodi
Regulation of the Saccharomyce ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
262
623-632
1999
1
-
1
-
1
-
-
-
2
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
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1
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1
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1
-
-
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-
2
1
-
1
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389632
Lodi
Transcriptional regulation of ...
Kluyveromyces lactis
Curr. Genet.
34
12-20
1998
-
-
1
-
1
-
-
-
1
-
-
1
-
3
-
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2
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1
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1
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1
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1
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2
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-
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-
-
-
389631
Lodi
Carbon catabolite repression i ...
Kluyveromyces lactis
Mol. Gen. Genet.
244
622-629
1994
-
-
1
-
-
-
-
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2
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1
-
4
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2
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1
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2
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389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
-
-
-
-
-
5
1
1
-
-
1
-
1
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1
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-
-
-
1
3
-
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-
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1
1
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4
-
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1
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-
4
-
-
-
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5
-
1
1
-
-
1
-
-
-
1
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-
-
1
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
389628
Olson
Purification and properties of ...
Megasphaera elsdenii
Biochemistry
18
4714-4723
1979
-
-
-
-
-
1
1
1
-
3
2
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
3
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389629
Olson
Suicide inactivation of the fl ...
Megasphaera elsdenii
Biochemistry
18
4724-4731
1979
-
-
-
-
-
-
2
-
-
1
-
1
-
1
-
-
-
-
-
-
-
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3
-
-
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-
-
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2
-
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-
-
2
-
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-
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2
-
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1
-
1
-
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-
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-
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287801
Hatefi
-
Metal-containing flavoprotein ...
Saccharomyces cerevisiae
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
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4
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4
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3
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389626
Brockman
D-lactate dehydrogenase of Pep ...
Megasphaera elsdenii
Methods Enzymol.
41B
309-312
1975
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1
3
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3
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1
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389623
Gregolin
The lactic dehydrogenase of ye ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
67
201-218
1963
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1
4
4
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1
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3
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3
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2
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389625
Nygaard
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Lactate dehydrogenases of yeas ...
Saccharomyces cerevisiae
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
7
557-565
1963
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389624
Nygaard
D(-)-lactic cytochrome c reduc ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
920-925
1961
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3
3
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5
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2
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2
3
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5
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