Cloned (Comment) | Organism |
---|---|
in Escherichia coli AR120 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
crystallization of Y143F mutant, vapor diffusion technique in presence of PEG 4000 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A289K | mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-lactate | - |
Saccharomyces cerevisiae | |
L-Mandelate | competitive | Saccharomyces cerevisiae | |
oxalate | - |
Saccharomyces cerevisiae | |
pyruvate | competitive at low concentrations, non-competitve at high concentrations | Saccharomyces cerevisiae | |
sulfite | competitive | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ferricyanide | Y254L mutant | Saccharomyces cerevisiae | |
0.04 | - |
ferricyanide | Y254F mutant | Saccharomyces cerevisiae | |
0.07 | - |
ferricyanide | wild type enzyme | Saccharomyces cerevisiae | |
0.22 | - |
L-Phenyllactate | wild type enzyme | Saccharomyces cerevisiae | |
0.29 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae | |
0.34 | - |
L-lactate | Y254F mutant | Saccharomyces cerevisiae | |
0.53 | - |
L-lactate | Y254L mutant | Saccharomyces cerevisiae | |
0.7 | - |
cytochrome c | Y254L mutant | Saccharomyces cerevisiae | |
1.43 | - |
L-Phenyllactate | R289K mutant enzyme | Saccharomyces cerevisiae | |
2.4 | - |
cytochrome c | Y254F mutant | Saccharomyces cerevisiae | |
131 | - |
cytochrome c | wild type enzyme | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial intermembrane space | soluble | Saccharomyces cerevisiae | 5758 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
4 * 57500, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-180°C, long term storage | Saccharomyces cerevisiae |
-80°C, 0.1 M phosphate buffer, 1 mM EDTA, 1 mM PMSF | Saccharomyces cerevisiae |
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + cytochrome c | - |
Saccharomyces cerevisiae | pyruvate + oxidized cytochrome c | - |
? | |
(S)-mandelate + ferricytochrome c | - |
Saccharomyces cerevisiae | hydroxy(phenyl)acetate + ferrocytochrome c | - |
? | |
(S)-phenyllactate + ferricytochrome c | - |
Saccharomyces cerevisiae | phenylpyruvate + ferrocytochrome c | - |
? | |
L-lactate + ferricyanide | - |
Saccharomyces cerevisiae | pyruvate + ferrocyanide + H+ | - |
? | |
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57500, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-lactate | Y254L mutant | Saccharomyces cerevisiae | |
7.5 | - |
L-lactate | Y254F mutant | Saccharomyces cerevisiae | |
155 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Saccharomyces cerevisiae | |
FMN | one FMN per subunit | Saccharomyces cerevisiae | |
heme | one heme per subunit | Saccharomyces cerevisiae | |
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
sulfite | - |
Saccharomyces cerevisiae | |
0.0026 | - |
L-Mandelate | - |
Saccharomyces cerevisiae | |
0.3 | - |
oxalate | - |
Saccharomyces cerevisiae | |
1.4 | - |
D-lactate | - |
Saccharomyces cerevisiae | |
3 | - |
pyruvate | competitive | Saccharomyces cerevisiae | |
30 | - |
pyruvate | non-competitive | Saccharomyces cerevisiae |