Crystallization (Comment) | Organism |
---|---|
used as essential purification step, crystallized as DNA complex | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
intact enzyme unstable at low ionic strength | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-lactate | - |
Saccharomyces cerevisiae | |
D-lactate | - |
Wickerhamomyces anomalus | |
L-lactate | 60% inhibition at 10 mM, no inhibition with cleaved form of enzyme | Saccharomyces cerevisiae | |
L-lactate | - |
Wickerhamomyces anomalus | |
oxalate | - |
Saccharomyces cerevisiae | |
oxalate | - |
Wickerhamomyces anomalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-lactate | - |
Wickerhamomyces anomalus | |
0.4 | - |
L-lactate | intact enzyme | Saccharomyces cerevisiae | |
1.3 | - |
L-lactate | - |
Wickerhamomyces anomalus | |
1.6 | - |
L-lactate | cleaved enzyme | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae | |
Fe2+ | bound to enzyme | Wickerhamomyces anomalus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
4 * 57000 | Saccharomyces cerevisiae |
60000 | - |
4 * 60000 | Wickerhamomyces anomalus |
220000 | - |
- |
Wickerhamomyces anomalus |
220000 | - |
intact enzyme | Saccharomyces cerevisiae |
230000 | - |
cleaved enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Wickerhamomyces anomalus | - |
pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Wickerhamomyces anomalus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Wickerhamomyces anomalus |
purification in presence and absence of PMSF results intact or cleaved enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
20°C, 0.2 M phosphate buffer, pH 7.2, 1 mM EDTA, protected from light, 50% loss of activity within 10-20 days | Wickerhamomyces anomalus |
under nitrogen, 1 month, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | - |
Wickerhamomyces anomalus | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Wickerhamomyces anomalus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000 | Saccharomyces cerevisiae |
tetramer | 4 * 60000 | Wickerhamomyces anomalus |
tetramer | 4 * 34000-36000 + 4 * 21000-22000, cleaved enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
cleaved enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one FMN per subunit | Saccharomyces cerevisiae | |
FMN | one FMN per subunit | Wickerhamomyces anomalus | |
heme | one heme per subunit | Saccharomyces cerevisiae | |
heme | one heme per subunit | Wickerhamomyces anomalus | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | |
additional information | electron acceptors other than ferricytochrome c used | Wickerhamomyces anomalus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
oxalate | intact enzyme | Saccharomyces cerevisiae | |
0.7 | - |
oxalate | - |
Wickerhamomyces anomalus | |
1.3 | - |
oxalate | cleaved enzyme | Saccharomyces cerevisiae | |
1.4 | - |
D-lactate | intact enzyme | Saccharomyces cerevisiae | |
5.3 | - |
D-lactate | - |
Wickerhamomyces anomalus | |
6 | - |
D-lactate | cleaved enzyme | Saccharomyces cerevisiae | |
45 | - |
L-lactate | - |
Wickerhamomyces anomalus |