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Literature summary for 1.1.2.3 extracted from

  • Jacq, C.; Lederer, F.
    Cytochrome b2 from bakers yeast (L-lactate dehydrogenase). A double-headed enzyme (1974), Eur. J. Biochem., 41, 311-320.
    View publication on PubMed
Search for more literature for 1.1.2.3

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
 4 * 57500, SDS-PAGE Saccharomyces cerevisiae
220000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
intact enzyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
4°C, 70% saturated ammonium sulfate, under nitrogen, 2 months, 20% loss of activity of purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 57500, SDS-PAGE Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN one FMN per subunit Saccharomyces cerevisiae
heme one heme per subunit Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae