Literature summary for 1.1.2.11 extracted from

Miyazaki, R.; Yamazaki, T.; Yoshimatsu, K.; Kojima, K.; Asano, R.; Sode, K.; Tsugawa, W.
Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase (2018), Bioelectrochemistry, 122, 115-122 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Agrobacterium fabrum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0034	-	cytochrome c	pH 7, 23°C	Agrobacterium fabrum
3.2	-	methyl alpha-D-glucoside	pH 7, 23°C	Agrobacterium fabrum

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium fabrum	A9CGJ1	i.e. catalytic subunit I, Gtd1	-
Agrobacterium fabrum C58	A9CGJ1	i.e. catalytic subunit I, Gtd1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic subunit I and G3DH hitch-hiker subunit II, purified by Ni2+ affinity chromatography followed by anion exchange chromatography	Agrobacterium fabrum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl alpha-D-glucoside + cytochrome c	-	Agrobacterium fabrum	methyl alpha-D-3-oxoglucoside + reduced cytochrome c	-	?
methyl alpha-D-glucoside + cytochrome c	-	Agrobacterium fabrum C58	methyl alpha-D-3-oxoglucoside + reduced cytochrome c	-	?
methyl alpha-D-glucoside + phenazine methosulfate	-	Agrobacterium fabrum	methyl alpha-D-3-oxoglucoside + reduced phenazine methosulfate	-	?
methyl alpha-D-glucoside + phenazine methosulfate	-	Agrobacterium fabrum C58	methyl alpha-D-3-oxoglucoside + reduced phenazine methosulfate	-	?

Subunits

Subunits	Comment	Organism
?	x * 63350, catalytic subunit I, calculated from sequence	Agrobacterium fabrum
More	enzyme is composed of the G3DH catalytic subunit I, G3DH hitch-hiker subunit II, and cytochrome c like molecule CYTc	Agrobacterium fabrum

Synonyms

Synonyms	Comment	Organism
Atu4377	-	Agrobacterium fabrum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Agrobacterium fabrum
[3Fe-4S]-center	the catalytic subunit contains an 3Fe-4S iron-sulfur cluster that acts as an electron acceptor for electrons from FAD in the oxidative half reaction	Agrobacterium fabrum

General Information

General Information	Comment	Organism
metabolism	In the catalytic subunit, the reductive half reaction occurs using FAD as the electron acceptor, and the 3Fe-4S iron sulfur cluster then accepts the electron from reduced FAD. The reduced iron-sulfur cluster transfers the electron to the external cytochrome c-like molecule	Agrobacterium fabrum

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Release 2023.1 (January 2023)