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Literature summary for 1.1.2.10 extracted from

  • Picone, N.; Op den Camp, H.J.
    Role of rare earth elements in methanol oxidation (2019), Curr. Opin. Chem. Biol., 49, 39-44 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
methanol pH and temperature not specified in the publication, lanthanide: Eu3+, Lu3+ Methylacidiphilum fumariolicum
0.0008
-
methanol pH and temperature not specified in the publication, lanthanide: La3+, Ce3+, Pr3+, Nd3+ Methylacidiphilum fumariolicum
0.0009
-
methanol pH and temperature not specified in the publication, lanthanide: Eu3+ Methylacidiphilum fumariolicum
0.0013
-
methanol pH and temperature not specified in the publication, lanthanide: Eu3+, La3+ Methylacidiphilum fumariolicum
0.029
-
methanol pH and temperature not specified in the publication, lanthanide: Ce3+ Bradyrhizobium sp.
0.029
-
methanol pH and temperature not specified in the publication, lanthanide: Nd3+ Methylorubrum extorquens
0.039
-
methanol pH and temperature not specified in the publication, lanthanide: Ce3+ Methylomonas sp. LW13
0.044
-
methanol pH and temperature not specified in the publication, lanthanide: La3+ Methylorubrum extorquens
0.055
-
methanol pH and temperature not specified in the publication, lanthanide: Ce3+ Methylotenera mobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Bradyrhizobium sp.
-
-
periplasm
-
Methylotenera mobilis
-
-
periplasm
-
Methylacidiphilum fumariolicum
-
-
periplasm
-
Methylomonas sp. LW13
-
-
periplasm
-
Methylorubrum extorquens
-
-
periplasm
-
Methylobacterium aquaticum
-
-
periplasm
-
Methylotuvimicrobium buryatense
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ce3+ lanthanide-dependent enzyme Bradyrhizobium sp.
Ce3+ lanthanide-dependent enzyme Methylotenera mobilis
Ce3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum
Ce3+ lanthanide-dependent enzyme Methylomonas sp. LW13
Eu3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum
La3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum
La3+ lanthanide-dependent enzyme Methylorubrum extorquens
Lu3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum
Nd3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum
Nd3+ lanthanide-dependent enzyme Methylorubrum extorquens
Pr3+ lanthanide-dependent enzyme Methylacidiphilum fumariolicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp.
-
-
-
Methylacidiphilum fumariolicum
-
-
-
Methylacidiphilum fumariolicum SolV
-
-
-
Methylobacterium aquaticum A0A0C6F7V8
-
-
Methylobacterium aquaticum 22A A0A0C6F7V8
-
-
Methylomonas sp. LW13
-
-
-
Methylorubrum extorquens C5B120
-
-
Methylotenera mobilis
-
-
-
Methylotenera mobilis JLW8
-
-
-
Methylotuvimicrobium buryatense A0A3F2YLY8
-
-
Methylotuvimicrobium buryatense 5G A0A3F2YLY8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + phenazine methosulfate
-
Bradyrhizobium sp. formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylotenera mobilis formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylacidiphilum fumariolicum formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylomonas sp. LW13 formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylorubrum extorquens formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylobacterium aquaticum formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylotuvimicrobium buryatense formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylacidiphilum fumariolicum SolV formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylobacterium aquaticum 22A formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylotuvimicrobium buryatense 5G formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Methylotenera mobilis JLW8 formaldehyde + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
homodimer
-
Bradyrhizobium sp.
homodimer
-
Methylotenera mobilis
homodimer
-
Methylacidiphilum fumariolicum
homodimer
-
Methylomonas sp. LW13
homodimer
-
Methylorubrum extorquens
homodimer
-
Methylobacterium aquaticum
homodimer
-
Methylotuvimicrobium buryatense

Synonyms

Synonyms Comment Organism
XoxF
-
Bradyrhizobium sp.
XoxF
-
Methylacidiphilum fumariolicum
XoxF
-
Methylotuvimicrobium buryatense
xoxF1
-
Methylorubrum extorquens
XoxF4-1
-
Methylotenera mobilis
XoxF4-2
-
Methylotenera mobilis
XoxF5
-
Methylomonas sp. LW13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bradyrhizobium sp.
7
-
-
Methylotenera mobilis
7
-
-
Methylacidiphilum fumariolicum
7
-
-
Methylomonas sp. LW13
7
-
-
Methylorubrum extorquens
7
-
-
Methylobacterium aquaticum
7
-
-
Methylotuvimicrobium buryatense

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Bradyrhizobium sp.
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylotenera mobilis
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylacidiphilum fumariolicum
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylomonas sp. LW13
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylorubrum extorquens
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylobacterium aquaticum
pyrroloquinoline quinone pyrroloquinoline quinone-containing enzyme Methylotuvimicrobium buryatense

Expression

Organism Comment Expression
Methylorubrum extorquens overexpression of xoxF at higher calcium concentrations compared to lanthanides up

General Information

General Information Comment Organism
malfunction xoxF is required for the expression of mxaF in Methylobacterium aquaticum 22A, since xoxF deletion mutants are not able to grow in the presence of calcium Methylobacterium aquaticum
metabolism xoxF is required for the expression of mxaF in Methylobacterium aquaticum 22A, since xoxF deletion mutants are not able to grow in the presence of calcium Methylobacterium aquaticum
metabolism XoxF is the preferred enzyme for methanol oxidation, even when calcium is present in 100fold higher concentrations than lanthanide Methylotuvimicrobium buryatense